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791741

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The synthesis and characterization of monodispersed chitosan-coated Fe3O4 nanoparticles via a facile one-step solvothermal process for adsorption of bovine serum albumin.

Mao Shen et al.

Nanoscale research letters, 9(1), 296-296 (2014-07-06)

Preparation of magnetic nanoparticles coated with chitosan (CS-coated Fe3O4 NPs) in one step by the solvothermal method in the presence of different amounts of added chitosan is reported here. The magnetic property of the obtained magnetic composite nanoparticles was confirmed

Enhanced uranium immobilization and reduction by Geobacter sulfurreducens biofilms.

Dena L Cologgi et al.

Applied and environmental microbiology, 80(21), 6638-6646 (2014-08-17)

Biofilms formed by dissimilatory metal reducers are of interest to develop permeable biobarriers for the immobilization of soluble contaminants such as uranium. Here we show that biofilms of the model uranium-reducing bacterium Geobacter sulfurreducens immobilized substantially more U(VI) than planktonic

Type 2 diabetes: increased expression and contribution of IKCa channels to vasodilation in small mesenteric arteries of ZDF rats.

Christian Schach et al.

American journal of physiology. Heart and circulatory physiology, 307(8), H1093-H1102 (2014-08-17)

Impaired endothelial function, which is dysregulated in diabetes, also precedes hypertension. We hypothesized that in Type 2 diabetes, the impaired endothelium-dependent relaxation is due to a loss of endothelium-derived hyperpolarization (EDH) that is regulated by impaired ion channel function. Zucker

Analysis of phthalates in milk and milk products by liquid chromatography coupled to quadrupole Orbitrap high-resolution mass spectrometry.

Wei Jia et al.

Journal of chromatography. A, 1362, 110-118 (2014-08-27)

A new analytical method was developed and validated for simultaneous analysis of 27 phthalates in milk and milk products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method. Ultrahigh-performance liquid

Rapid assessment of bioactive phenolics and methylxanthines in spent coffee grounds by FT-NIR spectroscopy.

Luís M Magalhães et al.

Talanta, 147, 460-467 (2015-11-26)

Spent coffee grounds (SCGs) are a great source of bioactive compounds with interest to pharmaceutical and cosmetic industries. Phenolics and methylxanthines are the main health related compounds present in SCG samples. Content estimation of these compounds in SCGs is of

Chitosan nanoparticles for siRNA delivery: optimization of processing/formulation parameters.

Elina Esmaeilzadeh Gharehdaghi et al.

Nucleic acid therapeutics, 24(6), 420-427 (2014-10-02)

Chitosan nanoparticles were prepared using ultrasonication methodology at specific amplitudes and times of sonication. Subsequently, small interfering RNA (siRNA) was added to the solution at predetermined values of nitrogen to phosphorous ratio (N/P), and stirring time. Employing response surfaces generated

Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.

Wen-Hsing Chen et al.

Bioprocess and biosystems engineering, 37(7), 1337-1344 (2013-12-19)

This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars. The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical

Intramuscular administration of a synthetic CpG-oligodeoxynucleotide modulates functional responses of neutrophils of neonatal foals.

Noah D Cohen et al.

PloS one, 9(10), e109865-e109865 (2014-10-22)

Neutrophils play an important role in protecting against infection. Foals have age-dependent deficiencies in neutrophil function that may contribute to their predisposition to infection. Thus, we investigated the ability of a CpG-ODN formulated with Emulsigen to modulate functional responses of

A simple method for patterning poly(dimethylsiloxane) barriers in paper using contact-printing with low-cost rubber stamps.

Karina Lopes Dornelas et al.

Analytica chimica acta, 858, 82-90 (2015-01-20)

This paper presents a simple and low-cost method for patterning poly(dimethylsiloxane) (PDMS) barriers in porous support such as paper for the construction of flexible microfluidic paper-based analytical devices (μPADs). The fabrication method consisted of contact-printing a solution of PDMS and

Additivity of the Specific Effects of Additives on Protein Phase Behavior.

Florian Platten et al.

The journal of physical chemistry. B, 119(48), 14986-14993 (2015-11-07)

Protein phase behavior and protein-protein interactions can be tuned by additives. We experimentally determined the phase behavior of lysozyme solutions, namely, the cloud-point temperature (CPT), in the presence of two additives, sodium chloride (NaCl) and guanidine hydrochloride (GuHCl). Their concentrations

Integration of phase separation with ultrasound-assisted salt-induced liquid-liquid microextraction for analyzing the fluoroquinones in human body fluids by liquid chromatography.

Huili Wang et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 985, 62-70 (2015-02-11)

Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted salt-induced liquid-liquid microextraction for determination of five fluoroquinones (FQs) in human body fluids. The integrated device consisted of three simple HDPE components used to separate the

Analytical method for simultaneous determination of bulk and intramolecular (13) C-isotope compositions of acetic acid.

Tarin Nimmanwudipong et al.

Rapid communications in mass spectrometry : RCM, 29(24), 2337-2340 (2015-11-14)

Headspace solid-phase microextraction (HS-SPME) combined with gas chromatography/pyrolysis-gas chromatography/combustion-isotope ratio mass spectrometry (GC/Py-GC/C-IRMS) was developed for the simultaneous determination of the intramolecular and molecular carbon-isotopic composition (δ(13) C value) of acetic acid. The δ(13) C values of carboxyl and methyl

Comparative study of alkaline hydrogen peroxide and organosolv pretreatments of sugarcane bagasse to improve the overall sugar yield.

Hailong Yu et al.

Bioresource technology, 187, 161-166 (2015-04-08)

Green liquor (GL) combined with H2O2 (GL-H2O2) and green liquor (GL) combined with ethanol (GL-ethanol) were chosen for treating sugarcane bagasse. Results showed that the glucose yield (calculated from the glucose content as a percentage of the theoretical glucose available

Short-chain fatty acids enhance adipocyte differentiation in the stromal vascular fraction of porcine adipose tissue.

Genlai Li et al.

The Journal of nutrition, 144(12), 1887-1895 (2014-10-17)

Short-chain fatty acids (SCFAs), including acetate, propionate, and butyrate, are the main products of microbial fermentation in the gut and might mediate some of the effects of gut microbiota and nutrition on development, metabolism, and pathogenesis of obesity and other

Infrapatellar fat pad-derived stem cells maintain their chondrogenic capacity in disease and can be used to engineer cartilaginous grafts of clinically relevant dimensions.

Yurong Liu et al.

Tissue engineering. Part A, 20(21-22), 3050-3062 (2014-05-03)

A therapy for regenerating large cartilaginous lesions within the articular surface of osteoarthritic joints remains elusive. While tissue engineering strategies such as matrix-assisted autologous chondrocyte implantation can be used in the repair of focal cartilage defects, extending such approaches to

Enzyme-based lignocellulose hydrolyzation--Sauter mean diameter of raw materials as a basis for cellulase performance characterization and yield prediction.

Robert Glaser

Journal of biotechnology, 214, 9-16 (2015-09-02)

The characterization of cellulase performance for industrial-scale processes holds special challenges. A huge gap exists between the enzyme characterization in a laboratory and large-scale process performances. Common cellulase mixtures from Novozymes and enzymes provided by Moscow State University were used

Rho kinase inhibition by fasudil in the striatal 6-hydroxydopamine lesion mouse model of Parkinson disease.

Lars Tatenhorst et al.

Journal of neuropathology and experimental neurology, 73(8), 770-779 (2014-07-09)

Chronic degeneration of nigrostriatal projections, followed by nigral dopaminergic cell death, is a key feature of Parkinson disease (PD). This study examines the neuroprotective potential of the rho kinase inhibitor fasudil in the 6-hydroxydopamine (6-OHDA) mouse model of PD in

Ultrasensitive quantification of serum estrogens in postmenopausal women and older men by liquid chromatography-tandem mass spectrometry.

Qingqing Wang et al.

Steroids, 96, 140-152 (2015-02-01)

An ultrasensitive stable isotope dilution liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed and validated for multiplexed quantitative analysis of six unconjugated and conjugated estrogens in human serum. The quantification utilized a new derivatization procedure, which formed analytes as pre-ionized

Cellular RNA is chemically modified by exposure to air pollution mixtures.

Kevin C Baldridge et al.

Inhalation toxicology, 27(1), 74-82 (2015-01-21)

RNAs are more susceptible to modifications than DNA, and chemical modifications in RNA have an effect on their structure and function. This study aimed to characterize chemical effects on total RNA in human A549 lung cells after exposure to elevated

Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8.

Stefano Fogal et al.

Applied microbiology and biotechnology, 99(22), 9541-9554 (2015-06-25)

Formate dehydrogenases (FDHs) are considered particularly useful enzymes in biocatalysis when the regeneration of the cofactor NAD(P)H is required, that is, in chiral synthesis with dehydrogenases. Their utilization is however limited to the recycling of NAD(+), since all (apart one)

The LIM homeodomain factor Lhx2 is required for hypothalamic tanycyte specification and differentiation.

Juan Salvatierra et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(50), 16809-16820 (2014-12-17)

Hypothalamic tanycytes, a radial glial-like ependymal cell population that expresses numerous genes selectively enriched in embryonic hypothalamic progenitors and adult neural stem cells, have recently been observed to serve as a source of adult-born neurons in the mammalian brain. The

Comparison of centrally injected tryptophan-related substances inducing sedation in acute isolation stress-induced neonatal chicks.

Junki Yoshida et al.

Pharmacology, biochemistry, and behavior, 129, 1-6 (2014-12-03)

In the present study, we first focused on the function of l-tryptophan (TRP) metabolites which are synthesized in different metabolic pathways, namely, the kynurenine (KYN) pathway and serotonin (5-HT) pathway during an acute isolation stress. When l-TRP metabolites were intracerebroventricularly

Highly Sensitive Aluminium(III) Ion Sensor Based on a Self-assembled Monolayer on a Gold Nanoparticles Modified Screen-printed Carbon Electrode.

Wong Pooi See et al.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 31(10), 997-1003 (2015-10-16)

A new approach for the development of a highly sensitive aluminium(III) ion sensor via the preconcentration of aluminium(III) ion with a self-assembled monolayer on a gold nanoparticles modified screen-printed carbon electrode and current mediation by potassium ferricyanide redox behavior during

Effect of addition of thermally modified cowpea protein on sensory acceptability and textural properties of wheat bread and sponge cake.

Lydia Campbell et al.

Food chemistry, 194, 1230-1237 (2015-10-17)

This paper investigates the sensory acceptability and textural properties of leavened wheat bread and sponge cake fortified with cow protein isolates that had been denatured and glycated by thermal treatment. Defatted cowpea flour was prepared from cow pea beans and

Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) for comparing betalain biosynthesis and antioxidant activity.

Dong Ho Suh et al.

Journal of agricultural and food chemistry, 62(34), 8764-8771 (2014-08-08)

Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) was performed using gas chromatography-time-of-flight-mass spectrometry and ultraperformance liquid chromatography-quadrupole-time-of-flight-mass spectrometry with multivariate analysis. Different species and parts of pitayas (red peel, RP; white peel, WP; red flesh

Comparison of sample preparation methods for the quantitative analysis of eicosanoids and other oxylipins in plasma by means of LC-MS/MS.

Annika I Ostermann et al.

Analytical and bioanalytical chemistry, 407(5), 1403-1414 (2014-12-30)

Oxylipins are potent lipid mediators. For the evaluation of their biological roles, several LC-MS based methods have been developed. While these methods are similar, the described sample preparation procedures for the extraction of oxylipins differ considerably. In order to deduce

Rapid measurement of tacrolimus in whole blood by paper spray-tandem mass spectrometry (PS-MS/MS).

Run-Zhang Shi et al.

Clinica chimica acta; international journal of clinical chemistry, 441, 99-104 (2014-12-30)

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) provides sensitivity and specificity for monitoring tacrolimus drug level in blood, but it requires an LC system and sample preparation, which is not amenable to random access testing typical of immunoassays. Paper spray (PS) ionization

Comprehensive analytical strategy for biomonitoring of pesticides in urine by liquid chromatography–orbitrap high resolution masss pectrometry.

M Roca et al.

Journal of chromatography. A, 1374, 66-76 (2014-12-17)

In this study we propose an analytical strategy that combines a target approach for the quantitative analysis of contemporary pesticide metabolites with a comprehensive post-target screening for the identification of biomarkers of exposure to environmental contaminants in urine using liquid

Bacteriophage PBC1 and its endolysin as an antimicrobial agent against Bacillus cereus.

Minsuk Kong et al.

Applied and environmental microbiology, 81(7), 2274-2283 (2015-01-18)

Bacillus cereus is an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. Due to the emergence of multidrug-resistant B. cereus strains, the demand for alternative therapeutic options is increasing. To address these problems, we isolated and characterized

Dynamic growth and shrinkage govern the pH dependence of RecA filament stability.

Sung Hyun Kim et al.

PloS one, 10(1), e0115611-e0115611 (2015-01-22)

RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined

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