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74564

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Jiří Danihlík et al.
Journal of chromatography. A, 1374, 134-144 (2014-12-02)
Apidaecins represent an important group of antimicrobial peptides occurring in honey bee hemolymph, where they play an important role as key components of humoral immunity. The present study demonstrates the development of a highly sensitive assay for apidaecin 1 isoforms...
Ming Lei et al.
Journal of pharmaceutical sciences, 104(3), 995-1004 (2014-12-30)
The biopharmaceutical industry increasingly demands thorough characterization of protein conformation and conformational dynamics to ensure product quality and consistency. Here, we present a chromatography-based method that is able to characterize protein conformation and conformational dynamics at peptide level resolution in...
Zijun Bie et al.
Analytica chimica acta, 834, 1-8 (2014-06-15)
Boronate affinity materials have attracted increasing attentions as sample enrichment platforms for glycoproteomic analysis in recent years. However, most of the boronate affinity materials that have already employed for proteomic analysis are suffering from apparent disadvantages, such as alkaline pH...
Rahul D Sheth et al.
Biotechnology and bioengineering, 111(8), 1595-1603 (2014-03-07)
In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based affinity precipitation process is developed for monoclonal antibody (mAb) purification from industrial harvest feeds. Greater than 99% mAb recoveries are obtained during the initial binding step of...
Marie-Claire Janin-Bussat et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 981-982, 9-13 (2015-01-18)
Antibody-drug conjugates (ADCs) are becoming a major class of oncology therapeutics. Because ADCs combine the monoclonal antibody specificity with the high toxicity of a drug, they can selectively kill tumor cells while minimizing toxicity to normal cells. Most of the...
Raquel Branquinho et al.
PloS one, 9(10), e110127-e110127 (2014-10-15)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical...
Wei Jia et al.
Journal of chromatography. A, 1362, 110-118 (2014-08-27)
A new analytical method was developed and validated for simultaneous analysis of 27 phthalates in milk and milk products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method. Ultrahigh-performance liquid...
Evelien A W Smits et al.
Journal of liposome research, 25(1), 46-57 (2014-07-02)
Besides the development of sample preparation methods for the determination of separate liposomal-encapsulated prednisolone phosphate and non-encapsulated prednisolone concentrations in murine plasma and blood, this article also presents the first description of an accurate sample preparation method for the determination...
Peiming Chen et al.
Journal of controlled release : official journal of the Controlled Release Society, 194, 341-349 (2014-09-16)
Macrophages are involved in a number of diseases, such as HIV infection/AIDS, tuberculosis, tumor development and atherosclerosis. Macrophages possess several cell surface receptors (e.g., the mannose receptor, MR) that may serve as drug delivery cellular portals for nanocarriers (NCs). In...
Wanyi Tai et al.
Biomaterials, 35(25), 7194-7203 (2014-05-31)
A graft copolymer with pendant drug segments can fold into nanostructures in a protein folding-like manner. The graft copolymer is constructed by directly polymerizing γ-camptothecin-glutamate N-carboxyanhydride (Glu(CPT)-NCA) on multiple sites of poly(ethylene glycol) (PEG)-based main chain via the ring open...
Masayoshi Okada et al.
Biochimica et biophysica acta, 1850(4), 657-666 (2014-12-09)
Arachnoid venoms contain numerous peptides with ion channel modifying and cytolytic activities. We developed a green fluorescent protein (GFP)-based assay that can monitor the changes in currents through overexpressed inwardly rectifying K(+) channels (Kir2.1), in which GFP expression was increased...
Diane Patricia Apie Gossan et al.
Phytochemistry, 109, 76-83 (2014-12-04)
Ten phenylalkyl-substituted iminosugars (1-10) and a cinnamic acid derived glucoside (11) were isolated from the roots of Glyphaea brevis (Malvaceae). Their structures were elucidated by 1D and 2D NMR analysis, as well as by HR-ESIMS. Compounds 1-10 retain an unprecedented...
Martyna Kielmas et al.
Analytical and bioanalytical chemistry, 407(9), 2557-2567 (2015-02-01)
Glycation of α-crystallin is responsible for age- and diabetic-related cataracts, which are the main cause of blindness worldwide. We optimized the method of identification of lysine residues prone to glycation using the combination of LC-MS, isotopic labeling, and modified synthetic...
Daryn Goodwin et al.
Pharmaceutical research, 32(5), 1570-1584 (2014-11-20)
In this study we aimed to address the poor drug-like properties of Gonadotropin-Releasing Hormone (GnRH) peptide through modification with lipids and carbohydrates. GnRH peptide was conjugated to 2-amino-D,L-octanoic acid (C8) and 2-amino-D,L-dodecanoic acid (C12) in monomer and dimer, along with...
Abbas M Walji et al.
ChemMedChem, 10(2), 245-252 (2014-12-04)
Developing new antiretroviral therapies for HIV-1 infection with potential for less frequent dosing represents an important goal within drug discovery. Herein, we present the discovery of ethyl (1-((4-((4-fluorobenzyl)carbamoyl)-1-methyl-2-(2-(5-methyl- 1,3,4-oxadiazole-2-carboxamido)propan-2-yl)-6-oxo-1,6-dihydropyrimidin-5-yl)oxy)ethyl) carbonate (MK-8970), a highly optimized prodrug of raltegravir (Isentress). Raltegravir is a...
Catherine Lavaud et al.
Carbohydrate research, 402, 225-231 (2014-12-17)
The chemical investigation of the methanolic extract from root bark of Zanha golungensis Hiern led to the isolation of five new and one known triterpenoid saponins. Their structures were elucidated by full analysis of their spectroscopic data and by partial...
Yu Huang et al.
Bioresource technology, 181, 224-230 (2015-02-07)
In this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in Gossypium barbadense and Gossypium hirsutum species. Sequential 1% H2SO4 pretreatment could further increase biomass digestibility of the steam-exploded...
Xiao-Lin Ruan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 965, 85-90 (2014-07-08)
In this study, magnetic single-walled carbon nanotubes (MSWCNTs) were prepared by impregnating magnetic Fe3O4 nanoparticles onto the surfaces of carboxylic single-walled carbon nanotubes based on electrostatic interactions. The prepared MSWCNTs were used as the adsorbent for the dispersive solid-phase extraction...
Stefan Opitz et al.
Analytical and bioanalytical chemistry, 407(4), 1107-1118 (2014-11-27)
Triton X-100 has been widely used in many analytical and preparative protocols for a long time. Nevertheless, mass spectrometry, chromatographic separation, and spectrophotometric readout may be considerably hampered by this detergent due to signal suppression, complex formation, and high blank...
Bradford A Paik et al.
Soft matter, 11(9), 1839-1850 (2015-01-23)
Polymer-peptide conjugates were produced via the copper-catalyzed azide-alkyne cycloaddition of poly(tert-butyl acrylate) (PtBA) and elastin-like peptides. An azide-functionalized polymer was produced via atom transfer radical polymerization (ATRP) followed by conversion of bromine end groups to azide groups. Subsequent reaction of...
Mazen Lee Hamad et al.
Pharmaceutical development and technology, 20(3), 314-319 (2013-12-04)
Levothyroxine sodium is an important medication used primarily for treating patients with hypothyroidism. Levothyroxine sodium tablets have been recalled many times since their 1955 introduction to the US market. These recalls resulted from the failure of lots to meet their...
Yulei Wang et al.
Analytica chimica acta, 840, 33-41 (2014-08-05)
In this work, a new stir cake sorptive extraction (SCSE) using polymeric ionic liquid monolith as sorbent was prepared. The sorbent was obtained by in situ copolymerization of an ionic liquid, 1-allyl-3-methylimidazolium bis[(trifluoro methyl)sulfonyl]imide (AMII) and divinylbenzene (DB) in the...
Xiao-Qiang Han et al.
PloS one, 9(7), e100380-e100380 (2014-07-12)
A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a...
Ulrich Lächelt et al.
Molecular pharmaceutics, 11(8), 2631-2639 (2014-04-24)
The antifolate drug methotrexate (MTX) can serve as a dual-functional ligand in antitumoral drug delivery, inducing both a folate receptor mediated cellular uptake and an intracellular cytotoxic action. Bioactivity of MTX however changes by conjugation; the activity can be affected...
Camila F Borgognoni et al.
Nanomedicine : nanotechnology, biology, and medicine, 11(2), 275-282 (2014-12-03)
The cytokine secretion of primary cells of human macrophages during the interaction of TiO2 nanoparticles (with an average primary size of 100-120 nm) is investigated down to concentration levels suggested to be relevant for in vivo conditions. We find that...
An-Dong Gong et al.
PloS one, 10(2), e0116871-e0116871 (2015-02-18)
Controlling toxigenic Fusarium graminearum (FG) is challenging. A bacterial strain (S76-3, identified as Bacillus amyloliquefaciens) that was isolated from diseased wheat spikes in the field displayed strong antifungal activity against FG. Reverse-phase high performance liquid chromatography and electrospray ionization mass...
Luke V Blakeway et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(12), 5197-5207 (2014-09-04)
Moraxella catarrhalis is a significant cause of otitis media and exacerbations of chronic obstructive pulmonary disease. Here, we characterize a phase-variable DNA methyltransferase (ModM), which contains 5'-CAAC-3' repeats in its open reading frame that mediate high-frequency mutation resulting in reversible...
Sophie T Williams et al.
Epigenetics, 9(12), 1596-1603 (2015-01-28)
The JmjC-domain-containing 2-oxoglutarate-dependent oxygenases catalyze protein hydroxylation and N(ϵ)-methyllysine demethylation via hydroxylation. A subgroup of this family, the JmjC lysine demethylases (JmjC KDMs) are involved in histone modifications at multiple sites. There are conflicting reports as to the substrate selectivity...
M A Patel et al.
Journal of chromatography. A, 1370, 240-245 (2014-12-03)
Near baseline separation of ten sulfated sodium salts of various structurally related estrogens employing a variety of bonded stationary phase packed columns was obtained using a conventional supercritical fluid chromatograph coupled with UV detection. Critical pairs 2/3 (8,9-dehydroestrone/17β-dihydroequilin) and 6/7...
Kevin M Legg et al.
Electrophoresis, 35(21-22), 3069-3078 (2014-07-30)
DNA profiling has transformed the field of forensic biology by making it possible to individualize biological stains. The identification of the stain itself, however, continues to present forensic serologists with significant challenges. Current antibody- and enzyme activity-based assays yield only...
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