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Christos D Georgiou et al.
Redox biology, 17, 128-142 (2018-04-24)
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
Sweta Rathore et al.
Journal of microencapsulation, 31(5), 469-478 (2014-04-05)
This work evaluated the feasibility of microencapsulating Clostridium acetobutylicum ATCC 824 cells by emulsification for fermentation to produce biobutanol. The effects of selected emulsification process on viability of the vegetative cells and spores were investigated to enable the selection of
Jesper T Andreasen et al.
Brain research, 1601, 117-126 (2015-01-13)
Depression and anxiety often co-occur, and conventional monoamine-facilitating antidepressants show efficacy against symptoms in both disorders. Rodent studies indicate that antidepressant effects of monoamine-based antidepressants involve increased α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid glutamate receptor (AMPAR) neurotransmission, and positive allosteric modulators (PAMs) at
Maiken Mellergaard et al.
The Journal of biological chemistry, 295(33), 11803-11821 (2020-07-02)
Staphylococcus aureus is among the leading causes of bacterial infections worldwide. The pathogenicity and establishment of S. aureus infections are tightly linked to its ability to modulate host immunity. Persistent infections are often associated with mutant staphylococcal strains that have
Daphne H E W Huberts et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(32), 11727-11731 (2014-07-30)
Calorie restriction (CR) is often described as the most robust manner to extend lifespan in a large variety of organisms. Hence, considerable research effort is directed toward understanding the mechanisms underlying CR, especially in the yeast Saccharomyces cerevisiae. However, the
Brooke Tata et al.
PLoS biology, 12(9), e1001952-e1001952 (2014-09-24)
Characterization of the genetic defects causing gonadotropic deficiency has made a major contribution to elucidation of the fundamental role of Kisspeptins and Neurokinin B in puberty onset and reproduction. The absence of puberty may also reveal neurodevelopmental disorders caused by
Jun Cheng et al.
Food chemistry, 168, 90-99 (2014-08-31)
The aim of this study was to investigate the applicability of artificial neural network (ANN) and multiple linear regression (MLR) models for the estimation of acrylamide reduction by flavonoids, using multiple antioxidant capacities of Maillard reaction products as variables via
Cicera R Lazzarotto et al.
Nature protocols, 13(11), 2615-2642 (2018-10-21)
Circularization for in vitro reporting of cleavage effects by sequencing (CIRCLE-seq) is a sensitive and unbiased method for defining the genome-wide activity (on-target and off-target) of CRISPR-Cas9 nucleases by selective sequencing of nuclease-cleaved genomic DNA (gDNA). Here, we describe a
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