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An in vitro model maintaining taxon-specific functional activities of the gut microbiome.

Leyuan Li et al.

Nature communications, 10(1), 4146-4146 (2019-09-14)

In vitro gut microbiome models could provide timely and cost-efficient solutions to study microbiome responses to drugs. For this purpose, in vitro models that maintain the functional and compositional profiles of in vivo gut microbiomes would be extremely valuable. Here

Comparative virulence characterization of the Shiga toxin phage-cured Escherichia coli O104:H4 and enteroaggregative Escherichia coli.

Nadja Haarmann et al.

International journal of medical microbiology : IJMM, 308(7), 912-920 (2018-06-27)

Escherichia coli O104:H4 (E. coli O104:H4), which caused in 2011 a massive foodborne outbreak in Germany, is characterized by an unusual combination of virulence traits. E. coli O104:H4 contains a prophage-encoded Shiga toxin (Stx) gene, which is the cardinal virulence

Evaluating in Vitro Culture Medium of Gut Microbiome with Orthogonal Experimental Design and a Metaproteomics Approach.

Leyuan Li et al.

Journal of proteome research, 17(1), 154-163 (2017-11-14)

In vitro culture based approaches are time- and cost-effective solutions for rapidly evaluating the effects of drugs or natural compounds against microbiomes. The nutritional composition of the culture medium is an important determinant for effectively maintaining the gut microbiome in

Inhibition of injured Escherichia coli by several selective agents.

D L Scheusner et al.

Applied microbiology, 21(1), 46-49 (1971-01-01)

A population of Escherichia coli ML30 cells was exposed to a quaternary ammonium compound, and injury to the cells was measured by a comparison of counts on Trypticase Soy Agar and Violet Red Bile Agar. Substantial injury could not be

In vitro assessment of the capacity of certain mycotoxin binders to adsorb some amino acids and water-soluble vitamins.

A Kihal et al.

Journal of dairy science, 103(4), 3125-3132 (2020-02-11)

The objective of this study was to evaluate the capacity of 6 mycotoxin binders (MTB) to adsorb 3 AA and 4 water-soluble vitamins (WSV). Two experiments were conducted in in vitro conditions to simulate postruminal digestion with pepsin, malic acid

The simulated in vitro infant gastrointestinal digestion of droplets covered with milk fat globule membrane polar lipids concentrate.

Jie Luo et al.

Journal of dairy science, 102(4), 2879-2889 (2019-02-11)

Milk fat globule membrane polar lipids (MPL) are increasingly used as the surface-active components for emulsions in many infant food products. However, the precise effect of the emulsifier MPL on the digestion of lipids during gastrointestinal digestion has not been

Complete Genomic Analysis of Enterococcus faecium Heat-Resistant Strain Developed by Two-Step Adaptation Laboratory Evolution Method.

Bonggyu Min et al.

Frontiers in bioengineering and biotechnology, 8, 828-828 (2020-08-15)

Stress resistance is an important trait expected of lactic acid bacteria used in food manufacturing. Among the various sources of stress, high temperature is a key factor that interrupts bacterial growth. In this regards, constant efforts are made for the

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Bacterial degradation of bile salts.

Philipp B

Applied Microbiology and Biotechnology, 89 (4), 903-915 (2011)

The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota.

Ilias Lagkouvardos et al.

Nature microbiology, 1(10), 16131-16131 (2016-09-28)

Intestinal bacteria influence mammalian physiology, but many types of bacteria are still uncharacterized. Moreover, reference strains of mouse gut bacteria are not easily available, although mouse models are extensively used in medical research. These are major limitations for the investigation

Effect of the adaptation to high bile salts concentrations on glycosidic activity, survival at low PH and cross-resistance to bile salts in Bifidobacterium.

Luis Noriega et al.

International journal of food microbiology, 94(1), 79-86 (2004-06-03)

Six derivatives with increased resistance to ox gall (MIC: > or = 1% w/v) and one derivative resistant to sodium cholate (MIC: 0.8% w/v) were obtained from more sensitive original Bifidobacterium strains. These microorganisms, and two additional cholate resistant derivatives

In Vitro Gastrointestinal Digestibility of Crystalline Oil-in-Water Emulsions: Influence of Fat Crystal Structure.

Wenjuan Jiao et al.

Journal of agricultural and food chemistry, 67(3), 927-934 (2019-01-05)

To investigate how the fat crystal structure affects lipid in vitro digestibility, 30% palm stearin-in-water emulsions were prepared after storage at different temperatures (4, 25, and 37 °C) for 1 h, which consisted of different polymorphic forms, sizes, and quantities

Bile salts regulate CYP7A1 expression and elicit a fibrotic response and abnormal lipid production in 3D liver microtissues.

Catherine Jane Messner et al.

Toxicology in vitro : an international journal published in association with BIBRA, 60, 261-271 (2019-06-14)

Disrupted regulation and accumulation of bile salts (BS) in the liver can contribute towards progressive liver damage and fibrosis. Here, we investigated the role of BS in the progression of cholestatic injury and liver fibrosis using 3D scaffold-free multicellular human

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