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Aqueous micro-contact printing of cell-adhesive biomolecules for patterning neuronal cell cultures.
Jang MJ, et al.
BioChip Journal, 6(2), 107-113 (2012)
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could
Ryan V Tien Sing Young et al.
Biomicrofluidics, 9(4), 046501-046501 (2015-07-17)
Liposomes are currently well-established as biocompatible delivery vehicles for numerous compounds. However, conventional manufacturing tends to rely on time-consuming processes, costly equipment, unstable reaction parameters, and numerous pre- and post-processing steps. Herein, we demonstrate a microscope-slide-sized alternative: a double flow-focusing
Ameya R Narkar et al.
Langmuir : the ACS journal of surfaces and colloids, 34(32), 9410-9417 (2018-07-24)
Up to 30 mol % of acrylic acid (AAc) was incorporated into a pH-responsive smart adhesive consisting of dopamine methacrylamide and 3-acrylamido phenylboronic acid. Fourier transform infrared spectroscopy and rheometry confirmed that the incorporation of AAc shifted the pH of
Clemens Kunz et al.
Nanomaterials (Basel, Switzerland), 10(6) (2020-06-24)
Despite intensive research activities in the field of laser-induced periodic surface structures (LIPSS), the large-area nanostructuring of glasses is still a challenging problem, which is mainly caused by the strongly non-linear absorption of the laser radiation by the dielectric material.
Dong Song et al.
Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 377(2138), 20180335-20180335 (2019-04-11)
Desert beetles and cactus plants collect water from fog in arid regions. The desert beetle uses heterogeneous wettability to transport water to its mouth. A cactus uses conical spines which provide Laplace pressure gradient to transport water to its base.
Heng Zhang et al.
Advanced materials (Deerfield Beach, Fla.), 27(45), 7420-7425 (2015-10-21)
Silicon gravure patterns are engineered to have cells that are wettable and lands that are not wettable by aqueous inks. This strategy allows excess ink on the lands to be removed without using a doctor blade. Using an aqueous silica
Bryan A Nerger et al.
Soft matter, 15(28), 5728-5738 (2019-07-04)
Type I collagen self-assembles into three-dimensional (3D) fibrous networks. These dynamic viscoelastic materials can be remodeled in response to mechanical and chemical signals to form anisotropic networks, the structure of which influences tissue development, homeostasis, and disease progression. Conventional approaches
Xander F van Kooten et al.
Scientific reports, 7(1), 10467-10467 (2017-09-07)
The use of on-chip isotachophoresis assays for diagnostic applications is often limited by the small volumes of standard microfluidic channels. Overcoming this limitation is particularly important for detection of 'discrete' biological targets (such as bacteria) at low concentrations, where the
Ying-Hua Chen et al.
Biomicrofluidics, 9(5), 054111-054111 (2015-10-22)
This paper reports a two-layered polydimethylsiloxane microfluidic device-Flip channel, capable of forming uniform-sized embryoid bodies (EBs) and performing stem cell differentiation within the same device after flipping the microfluidic channel. The size of EBs can be well controlled by designing
Zhen Yuan et al.
Advanced materials (Deerfield Beach, Fla.), 32(21), e1908385-e1908385 (2020-04-15)
Multiplexed gas detection at room temperature is critical for practical applications, such as for tracking the complex chemical environments associated with food decomposition and spoilage. An integrated array of multiple silicon-based, chemical-sensitive field effect transistors (CSFETs) is presented to realize
Mallory L Hinks et al.
Physical chemistry chemical physics : PCCP, 18(13), 8785-8793 (2015-12-22)
This work explores the effect of environmental conditions on the photodegradation rates of atmospherically relevant, photolabile, organic molecules embedded in a film of secondary organic material (SOM). Three types of SOM were studied: α-pinene/O3 SOM (PSOM), limonene/O3 SOM (LSOM), and
Yixuan Ming et al.
Frontiers in neuroscience, 14, 563905-563905 (2020-10-31)
Studies aimed at neurological drug discovery have been carried out both in vitro and in vivo. In vitro cell culture models have showed potential as drug testing platforms characterized by high throughput, low cost, good reproducibility and ease of handling
Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device
Tenstad Ellen, et al.
Lab on a chip, 10(11), 1401-1409 (2010)
Biomimetic polyimide nanotube arrays with slippery or sticky superhydrophobicity
Zhu S, et al.
Journal of Colloid and Interface Science, 344(2), 541-546 (2010)
Andrea Manfrin et al.
Nature methods, 16(7), 640-648 (2019-06-30)
Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hPSC) colonies to spatiotemporally
Huiyan Li et al.
Scientific reports, 5, 11688-11688 (2015-07-08)
The coordinated delivery of minute amounts of different reagents is important for microfluidics and microarrays, but is dependent on advanced equipment such as microarrayers. Previously, we developed the snap chip for the direct transfer of reagents, thus realizing fluidic operations
Soluble surfactant spreading on spatially confined thin liquid films
Hanyak M, et al.
Soft Matter, 8(29), 7660-7671 (2012)
Akio Suzuki et al.
PloS one, 14(6), e0217944-e0217944 (2019-06-07)
Anti-prion protein (PrP) monoclonal antibody 132, which recognizes mouse PrP amino acids 119-127, enables us to reliably detect abnormal isoform prion protein (PrPSc) in cells or frozen tissue sections by immunofluorescence assay, although treatment with guanidinium salts is a prerequisite.
Celal Con et al.
Nanoscale research letters, 8(1), 394-394 (2013-09-26)
Polydimethylsiloxane (PDMS) is the most popular and versatile material for soft lithography due to its flexibility and easy fabrication by molding process. However, for nanoscale patterns, it is challenging to fill uncured PDMS into the holes or trenches on the
Wei-Hung Jung et al.
Cells, 8(8) (2019-08-07)
It has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature at the cellular and multicellular levels. To characterize and determine the mechanisms of curvature-dependent cell
Zhenglin Li et al.
Science advances, 5(4), eaat3080-eaat3080 (2019-04-25)
Control of periodic sequential flows of multisolutions is invaluable in a variety of technology and science applications, but it requires complex and expensive external controllers. Here, we present microfluidic systems that autonomously regulate periodic sequential flows without any user instructions
Zhen Zhu et al.
Micromachines, 10(3) (2019-03-03)
The development of miniaturized devices for studying zebrafish embryos has been limited due to complicated fabrication and operation processes. Here, we reported on a microfluidic device that enabled the capture and culture of zebrafish embryos and real-time monitoring of dynamic
Eui Hyuk Kim et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(13), 1802351-1802351 (2019-08-06)
In addition to the demand for stimuli-responsive sensors that can detect various vital signals in epidermal skin, the development of electronic skin displays that quantitatively detect and visualize various epidermal stimuli such as the temperature, sweat gland activity, and conductance
Zhiwei He et al.
Soft matter, 13(37), 6562-6568 (2017-09-13)
Preventing icing on exposed surfaces is important for life and technology. While suppressing ice nucleation by surface structuring and local confinement is highly desirable and yet to be achieved, a realistic roadmap of icephobicity is to live with ice, but
Sungjune Park et al.
Small (Weinheim an der Bergstrasse, Germany), 14(20), e1704460-e1704460 (2018-04-17)
Buckling instabilities generate microscale features in thin films in a facile manner. Buckles can form, for example, by heating a metal/polymer film stack on a rigid substrate. Thermal expansion differences of the individual layers generate compressive stress that causes the
Jonathan H Dwyer et al.
Langmuir : the ACS journal of surfaces and colloids, 35(38), 12492-12500 (2019-08-29)
Semiconducting single-walled carbon nanotube (s-CNT) arrays are being explored for next-generation semiconductor electronics. Even with the multitude of alignment and spatially localized s-CNT deposition methods designed to control s-CNT deposition, fundamental understanding of the driving forces for s-CNT deposition is
Aqueous micro-contact printing of cell-adhesive biomolecules for patterning neuronal cell cultures.
Jang MJ, et al.
Journal of Neural Engineering, 9(4), 107-113 (2012)
Yu-Heng Cheng et al.
Nature communications, 10(1), 2163-2163 (2019-05-17)
Molecular analysis of circulating tumor cells (CTCs) at single-cell resolution offers great promise for cancer diagnostics and therapeutics from simple liquid biopsy. Recent development of massively parallel single-cell RNA-sequencing (scRNA-seq) provides a powerful method to resolve the cellular heterogeneity from
Jinxin Zhang et al.
Lab on a chip, 19(6), 984-992 (2019-02-16)
Controllable, precise, and stable rotational manipulation of model organisms is valuable in many biomedical, bioengineering, and biophysics applications. We present an acoustofluidic chip capable of rotating Caenorhabditis elegans (C. elegans) in both static and continuous flow in a controllable manner.
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