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Dylan S Mackay et al.
The American journal of clinical nutrition, 101(3), 432-439 (2015-03-04)
Benefits of plant sterols (PS) for cholesterol lowering are compromised by large variability in efficacy across individuals. High fractional cholesterol synthesis measured by deuterium incorporation has been associated with nonresponse to PS consumption; however, prospective studies that show this association
Ceren Kutlu et al.
Journal of microencapsulation, 31(7), 700-707 (2014-06-26)
The chitosan scaffold, which has both of anticancer and antivascularization effects, was developed for using in local therapy of brain tumours. This is why, poly-lactic-co-glycolic acid (50:50) nanoparticles (~200 nm) including an anticancer drug, 5-fluorouracil (5-FU), were prepared by emulsion-solvent
Roghayeh Imani et al.
Nanoscale research letters, 10(1), 441-441 (2015-11-18)
The applications of zinc oxide (ZnO) nanowires (NWs) in implantable wireless devices, such as diagnostic nanobiosensors and nanobiogenerators, have recently attracted enormous attention due to their unique properties. However, for these implantable nanodevices, the biocompatibility and the ability to control the
Siyoung Choi et al.
Acta biomaterialia, 24, 333-342 (2015-06-15)
Breast microcalcifications are routinely explored for mammographic detection of breast cancer and are primarily composed of non-stoichiometric hydroxyapatite (Ca10-x(PO4)6-x(CO3)x(OH)2-x) (HA). Interestingly, HA morphology and carbonate substitution vary in malignant vs. benign lesions. However, whether or not these changes (i) are
Marion Gabel et al.
The Journal of cell biology, 210(5), 785-800 (2015-09-02)
Annexin A2, a calcium-, actin-, and lipid-binding protein involved in exocytosis, mediates the formation of lipid microdomains required for the structural and spatial organization of fusion sites at the plasma membrane. To understand how annexin A2 promotes this membrane remodeling
Ying Zhang et al.
Biomaterials, 64, 88-97 (2015-07-01)
Autologous stem cells are a promising cell source for cartilage regeneration; however, cell replicative senescence and joint posttraumatic inflammation provide challenges in bringing this treatment modality to fruition. In this study, we hypothesized that preconditioning with p38 MAPK inhibitor (sb203580)
G Chiavari et al.
Journal of chromatography. A, 922(1-2), 235-241 (2001-08-07)
Alpha-amino acids were pyrolysed at 600 degrees C in the presence of hexamethyldisilazane (HMDS) and the formed volatile products were analysed on line by gas chromatography-mass spectrometry (GC-MS). Glycine, alanine, valine, leucine, isoleucine, norleucine, methionine, phenylalanine yielded principally the trimethylsilyl
Farzad Foroutan et al.
Biomedical materials (Bristol, England), 10(4), 045025-045025 (2015-08-27)
In the present study, we report a new and facile sol-gel synthesis of phosphate-based glasses with the general formula of (P2O5)55-(CaO)25-(Na2O)(20-x)-(TiO2) x , where x = 0, 5, 10 or 15, for bone tissue engineering applications. The sol-gel synthesis method
Nina Parmar et al.
Tissue engineering. Part C, Methods, 21(4), 404-412 (2014-09-11)
Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are
Qamar Zia et al.
International journal of nanomedicine, 10, 1769-1790 (2015-03-19)
In the present study, we developed a self-assembled biodegradable polyglutamic acid (PGA)-based formulation of amphotericin B (AmB) and evaluated its in vitro antifungal potential against Candida albicans. The AmB-loaded PGA nanoparticles were prepared in-house and had a mean size dimension
Christoph Schell et al.
PloS one, 10(6), e0129424-e0129424 (2015-06-16)
The coxsackie- and adenovirus receptor (CXADR) is a member of the immunoglobulin protein superfamily, present in various epithelial cells including glomerular epithelial cells. Beside its known function as a virus receptor, it also constitutes an integral part of cell-junctions. Previous
Marcia Shu-Wei Su et al.
Microbiology (Reading, England), 160(Pt 4), 795-806 (2014-02-11)
This study characterized the two-component regulatory systems encoded by bfrKRT and cemAKR, and assessed their influence on biofilm formation by Lactobacillus reuteri 100-23. A method for deletion of multiple genes was employed to disrupt the genetic loci of two-component systems.
Elsa Vennat et al.
Dental materials : official publication of the Academy of Dental Materials, 25(6), 729-735 (2009-01-29)
The objectives of this study were to assess demineralized dentin porosity and quantify the different porous features distribution within the material using mercury intrusion porosimetry (MIP) technique. We compared hexamethyldisilazane (HMDS) drying and lyophilization (LYO) (freeze-drying) in sample preparation. Fifty-six
S V Buravkov et al.
Bulletin of experimental biology and medicine, 151(3), 378-382 (2012-03-28)
We compared conventional method for specimen (cell cultures, tissue specimens) preparation for scanning electron microscopy and a method without sputtering and critical-point drying. OTO-method (osmium-thiocarbohydrazide-osmium) with sample impregnation with hexamethyldisilazane followed by air drying was used as an alternative method.
Young-Jae Jo et al.
Journal of periodontal & implant science, 41(2), 67-72 (2011-05-11)
The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Rat bone marrow derived
Luigi Iuliano et al.
Steroids, 99(Pt B), 189-193 (2015-02-11)
The human brain contains approximately 25% of the body's cholesterol. The brain is separated from the circulation by the blood brain barrier. While cholesterol will not passes this barrier, oxygenated forms of cholesterol can cross the barrier. Here by measuring
Jie-Ren Li et al.
ACS nano, 9(7), 6738-6746 (2015-06-10)
A recent finding reports that co-stimulation of the high-affinity immunoglobulin E (IgE) receptor (FcεRI) and the chemokine receptor 1 (CCR1) triggered formation of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation was attained using corresponding ligands: IgE binding antigen and
Alba C De Luca et al.
Journal of biomedical materials research. Part A, 103(8), 2689-2700 (2015-01-30)
Microgrooved surfaces have been used extensively to influence cell contact guidance. Guiding cell growth, extracellular matrix deposition, and mineralization is important for bone implant longevity. In this study, we investigated the osteoblast response to microgrooved metallic surfaces in serum-supplemented medium.
Matthew J Lawrence et al.
Atherosclerosis, 240(2), 402-407 (2015-04-19)
Changes in clot microstructure are increasingly implicated in the pathology of atherosclerosis although most data are from techniques in the remote laboratory using altered blood. We validate the novel biomarker Gel Point in STEMI patients and assess therapeutic interventions. Gel
Mahdis Shayan et al.
Journal of materials science. Materials in medicine, 25(12), 2721-2730 (2014-07-31)
Osteoblast response was evaluated with polymethylmethacrylate (PMMA)/titanium dioxide (TiO2) nanocomposite thin films that exhibit the controllable wettability with ultraviolet (UV) treatment. In this study, three samples of PMMA/TiO2 were fabricated with three different compositional volume ratios (i.e., 25/75, 50/50, and
Mario Janjetovic et al.
Chemical communications (Cambridge, England), 49(18), 1826-1828 (2013-01-30)
Sm(HMDS)(2) in n-hexane mediates fast cleavage of primary, secondary and tertiary alkyl fluorides in good to excellent yields. In n-hexane Sm(HMDS)(2) exhibits uniquely enhanced reductive ability towards the C-F bond compared to when using THF as solvent.
N L Davison et al.
European cells & materials, 29, 314-329 (2015-06-21)
It has been reported that surface microstructural dimensions can influence the osteoinductivity of calcium phosphates (CaPs), and osteoclasts may play a role in this process. We hypothesised that surface structural dimensions of ≤ 1 μm trigger osteoinduction and osteoclast formation
Benjamin S Spearman et al.
Acta biomaterialia, 28, 109-120 (2015-09-27)
Conductive and electroactive polymers have the potential to enhance engineered cardiac tissue function. In this study, an interpenetrating network of the electrically-conductive polymer polypyrrole (PPy) was grown within a matrix of flexible polycaprolactone (PCL) and evaluated as a platform for
Sascha Nowak et al.
Analytical and bioanalytical chemistry, 407(3), 1023-1026 (2014-09-23)
Low gas flow ICP-OES with a total argon consumption below 0.7 L/min is introduced for the analysis of trace elements in blood samples to investigate the influence of samples containing an organic solvent in a demanding matrix on the performance of
Jessica L Terrell et al.
Nature communications, 6, 8500-8500 (2015-10-13)
Advances in nanotechnology have provided unprecedented physical means to sample molecular space. Living cells provide additional capability in that they identify molecules within complex environments and actuate function. We have merged cells with nanotechnology for an integrated molecular processing network.
Tong Zhang et al.
Electrophoresis, 29(4), 944-951 (2008-01-24)
This simultaneous separation of basic, acidic and neutral analytes by pressure-assisted CEC (pCEC) using a hybrid (tetramethoxysilane and methyltrimethoxysilane) silica-based monolith, chemically modified with octadecyldimethylchlorosilane followed by endcapping with hexamethyldisilazane is described. The endcapping resulted in near Gaussian peaks for
Tian Xia et al.
Applied and environmental microbiology, 81(10), 3387-3394 (2015-03-10)
Escherichia coli that is unable to metabolize d-glucose (with knockouts in ptsG, manZ, and glk) accumulates a small amount of d-glucose (yield of about 0.01 g/g) during growth on the pentoses d-xylose or l-arabinose as a sole carbon source. Additional
Jennifer A Lee et al.
Journal of biomedical materials research. Part A, 103(10), 3188-3200 (2015-03-18)
We have used the additive manufacturing technology of selective laser sintering (SLS), together with post SLS heat treatment, to produce porous three dimensional scaffolds from the glass-ceramic apatite-wollastonite (A-W). The A-W scaffolds were custom-designed to incorporate a cylindrical central channel
Chaganti Srinivasa Reddy et al.
Journal of biomedical materials research. Part A, 102(10), 3713-3725 (2013-11-30)
Polycaprolactone (PCL), a synthetic biocompatible and biodegradable polymer generally used as a scaffold material for tissue engineering applications. The high stiffness and hydrophobicity of the PCL fiber mesh does not provide significant cell attachment and proliferation in cardiac tissue engineering.
Hsin-Yi Lin et al.
Journal of biomaterials science. Polymer edition, 26(13), 855-867 (2015-07-15)
A tri-layered chitosan-based scaffold was successfully made to replicate the striation of a full-thickness skin more accurately than a single- or bi-layered scaffold, which needed weeks of co-culturing of fibroblasts and keratinocytes to achieve similar striation. Chitosan solution was freeze-dried
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