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B-cell-associated immune profiles in patients with decompensated cirrhosis.

Joo Yeon Jhun et al.

Scandinavian journal of gastroenterology, 50(7), 884-891 (2015-04-12)

Previous observations on immune dysfunction in decompensated cirrhosis have raised the possibility of B-cell impairment. B-cell subsets in decompensated cirrhotic patients were investigated. Twenty-six decompensated cirrhotic patients and 26 healthy controls were included in this study. The percentages of B-cell

Determination method for mono- and diethanolamine in workplace air by high-performance liquid chromatography.

Akito Takeuchi et al.

Journal of occupational health, 54(4), 340-343 (2012-07-21)

The purpose of this research was to develop a simultaneous determination method for monoethanol-amine (MEA) and diethanolamine (DEA) in workplace air for risk assessment. The characteristics of the proposed method, such as recovery, quantitation limit, reproducibility and storage stability of

Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization.

Xu Yu et al.

Biosensors & bioelectronics, 66, 520-526 (2014-12-17)

A novel highly sensitive colorimetric assay for DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization was established. The DNA modified superparamagnetic beads were demonstrated to capture and enrich the target DNA in the hybridization

Decrease of immature B cell and interleukin-10 during early-post-transplant period in renal transplant recipients under tacrolimus based immunosuppression.

Byung Ha Chung et al.

Transplant immunology, 30(4), 159-167 (2014-04-09)

B lymphocytes are known to play a role in kidney transplantation (KT) outcomes. Here, we evaluated the proportion of B cell subsets before and after KT. Twenty-one patients, who showed stable allograft function without acute rejection in the month following

Ion-pairing reversed-phase liquid chromatography fractionation in combination with isotope labeling reversed-phase liquid chromatography-mass spectrometry for comprehensive metabolome profiling.

Kevin Guo et al.

Journal of chromatography. A, 1218(23), 3689-3694 (2011-05-06)

We report a novel two-dimensional (2D) separation strategy aimed at improving the detectability of liquid chromatography mass spectrometry (LC-MS) for metabolome analysis. It is based on the use of ion-pairing (IP) reversed-phase (RP) LC as the first dimension separation to

Half-integer spin heptanuclear single-molecule magnet with an unusual Mn(III)6Mn(II) exchange-coupled core.

Siou-Yin Chen et al.

Inorganic chemistry, 51(8), 4448-4457 (2012-04-05)

The synthesis, X-ray crystallography, magnetic properties, and high-field electron paramagnetic resonance (HFEPR) of a new heptanuclear manganese complex [Mn(7)(heamp)(6)](ClO(4))(2)·4CH(2)Cl(2)·H(2)O (complex 2), in which heampH(3) is 2-[N,N-di(2-hydroxyethyl)aminomethyl]phenol (compound 1), is reported. Complex 2 has a hexagonal, disk-shaped topology and contains six

Developing a preventive immunization approach against insect bite hypersensitivity using recombinant allergens: A pilot study.

Sigridur Jonsdottir et al.

Veterinary immunology and immunopathology, 166(1-2), 8-21 (2015-05-26)

Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses caused by bites of midges (Culicoides spp.). IgE-mediated reactions are often involved in the pathogenesis of this disease. IBH does not occur in Iceland due to the absence of Culicoides

A validated chiral LC method for enantiomeric separation of nebivolol stereoisomers in bulk drugs and dosage forms on amylose-based stationary phase.

Karri Visweswara Rao et al.

Journal of chromatographic science, 52(9), 1051-1058 (2013-10-31)

A novel and reproducible isocratic normal phase liquid chromatographic method was developed for the quantitative determination of 10 stereoisomers of Nebivolol in pharmaceutical bulk drugs and dosage forms. The method was developed using an amylose-based chiral stationary phase, Chiralpak AD-3

Antimicrobial action of N-(n-dodecyl)diethanolamine on Escherichia coli: effects on enzymes and growing cultures.

A R W Smith et al.

Journal of applied microbiology, 105(6), 2161-2168 (2009-01-06)

This study investigates the effects of N-(n-dodecyl)diethanolamine (DDA) on enzymes and growing cells of Escherichia coli NCIMB 8277. Enzyme activities in the presence of DDA were determined by measuring substrate-dependent oxygen consumption by whole cells, or of NADH formation or

Antibodies to early EBV, CMV, and HHV6 antigens in systemic lupus erythematosus patients.

N S Rasmussen et al.

Scandinavian journal of rheumatology, 44(2), 143-149 (2015-01-07)

We investigated the antibody levels against early antigens of Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus 6 (HHV6) in systemic lupus erythematosus (SLE) patients and healthy controls, and further correlated these antibodies to haematology/biochemistry, serology, and disease activity measures.

Assessment of the impact of manufacturing changes on the physicochemical properties and biological activity of Her1-ECD vaccine during product development.

Katia Garcia Duardo et al.

Vaccine, 33(35), 4292-4299 (2015-05-25)

Vaccine preparations based on the extracellular domain of Her1 (Her1-ECD) have demonstrated, in vitro and in vivo, a potent antimetastatic effect on EGFR(+) Lewis lung carcinoma model, while associated side effects were absent. The Her1-ECD is a glycoprotein with a

Modelling the sulfoxygenation activity of vanadate-dependent peroxidases.

Pingsong Wu et al.

Chemistry & biodiversity, 5(10), 1913-1926 (2008-10-31)

Vanadate-dependent peroxidases contain, in their active center, vanadate covalently attached to histidine in an overall trigonal-bipyramidal array. We describe here the synthesis and characterization of optically active amino alcohols and their vanadium(V) complexes, and we show that the structural models

Deimination level and peptidyl arginine deiminase 2 expression are elevated in astrocytes with increased incubation temperature.

Mabel Enriquez-Algeciras et al.

Journal of neuroscience research, 93(9), 1388-1398 (2015-03-25)

Astrocytes respond to environmental cues, including changes in temperatures. Increased deimination, observed in many progressive neurological diseases, is thought to be contributed by astrocytes. We determined the level of deimination and expression of peptidyl arginine deiminase 2 (PAD2) in isolated

Gliotoxin potentiates osteoblast differentiation by inhibiting nuclear factor-κB signaling.

Guangye Wang et al.

Molecular medicine reports, 12(1), 877-884 (2015-03-31)

The differentiation of pluripotent mesenchymal stem cells to mature osteoblasts is crucial for the maintenance of the adult skeleton. In rheumatic arthritis, osteoblast differentiation is impaired by the overproduction of cytokine tumor necrosis factor (TNF)‑α. It has been demonstrated that

Antibody-mediated immune suppression of erythrocyte alloimmunization can occur independently from red cell clearance or epitope masking in a murine model.

Honghui Yu et al.

Journal of immunology (Baltimore, Md. : 1950), 193(6), 2902-2910 (2014-08-15)

Anti-D can prevent immunization to the RhD Ag on RBCs, a phenomenon commonly termed Ab-mediated immune suppression (AMIS). The most accepted theory to explain this effect has been the rapid clearance of RBCs. In mouse models using SRBC, these xenogeneic

Sequestration of free cholesterol in cell membranes by prions correlates with cytoplasmic phospholipase A2 activation.

Clive Bate et al.

BMC biology, 6, 8-8 (2008-02-14)

The transmissible spongiform encephalopathies (TSEs), otherwise known as the prion diseases, occur following the conversion of the normal cellular prion protein (PrPC) to an alternatively folded isoform (PrPSc). The accumulation of PrPSc within the brain leads to neurodegeneration through an

The reaction between CO₂ and diethanolamine at 298 K.

Versteeg GF and Oyevaar MH.

Chemical Engineering Science, 44(5), 1264-1268 (1989)

Intra- and intermolecular interaction ECL study of novel ruthenium tris-bipyridyl complexes with different amine reductants.

Shiguo Sun et al.

Dalton transactions (Cambridge, England : 2003), (38)(38), 7969-7974 (2009-09-23)

A series of ruthenium(II) tris-bipyridyl complexes covalently linked with different amine reductants such as tripropylamine (TPrA), ethanolamine and diethanolamine for an electrochemiluminescence (ECL) system have been synthesized. Their ECL property at different working electrodes has been studied with and without

Harmonization of values for serum alkaline phosphatase catalytic activity concentration employing commutable calibration materials.

Giampaolo Cattozzo et al.

Clinica chimica acta; international journal of clinical chemistry, 411(11-12), 882-885 (2010-03-17)

Harmonization of results allows a more effective utilization of laboratory tests; we verified the feasibility of harmonizing serum alkaline phosphatase results by two methods. Patient sera (n=106) and candidate calibration materials (n=8) were analyzed by two methods, employing either diethanolamine

Seawater ecotoxicity of monoethanolamine, diethanolamine and triethanolamine.

G Libralato et al.

Journal of hazardous materials, 176(1-3), 535-539 (2009-12-22)

Monoethanolamine (MEA), diethanolamine (DEA) and triethanolamine (TEA) are compounds with potential acute, sub-chronic and chronic toxicity effects towards aquatic species. A literature review highlighted the existence of a gap in the knowledge on their toxicity with saltwater testing species. A

IL-21-Expressing Mesenchymal Stem Cells Prevent Lethal B-Cell Lymphoma Through Efficient Delivery of IL-21, Which Redirects the Immune System to Target the Tumor.

Nayoun Kim et al.

Stem cells and development, 24(23), 2808-2821 (2015-09-29)

Interleukin (IL)-21, a proinflammatory cytokine, has been developed as an immunotherapeutic approach due to its effects on various lymphocytes, including natural killer (NK) cells and T cells; however, the clinical success in cancer patients has been limited. Recently, mesenchymal stem

Chlamydial lung infection induces transient IL-9 production which is redundant for host defense against primary infection.

Ying Peng et al.

PloS one, 10(2), e0115195-e0115195 (2015-02-04)

IL-9/Th9 responses are recently found to be important for innate and adaptive immunity particularly in parasitic infections. To date, the study on the role of IL-9 in bacterial infections is limited and the reported data are contradictory. One reported function

Qualitative differences in brain-infiltrating T cells are associated with a fatal outcome in mice infected with Japanese encephalitis virus.

Kenji Shirai et al.

Archives of virology, 160(3), 765-775 (2015-01-22)

Japanese encephalitis (JE) is the most important form of viral encephalitis in Asia. The critical factors determining mortality and severity of JE virus (JEV) infection remain unclear. We identified brain-infiltrating T cells associated with a fatal outcome of JEV infection

Activated group 3 innate lymphoid cells promote T-cell-mediated immune responses.

Nicole von Burg et al.

Proceedings of the National Academy of Sciences of the United States of America, 111(35), 12835-12840 (2014-08-20)

Group 3 innate lymphoid cells (ILC3s) have emerged as important cellular players in tissue repair and innate immunity. Whether these cells meaningfully regulate adaptive immune responses upon activation has yet to be explored. Here we show that upon IL-1β stimulation

Thin-film assembly of diethanolamine-based lipidic material as potential gene carrier in mouse embryonic neural stem cells.

Ken-Ichi Kusumoto et al.

Journal of biomedical materials research. Part A, 91(1), 1-10 (2008-08-07)

Understanding of lipidic materials used for gene delivery system is essential for the effective design and development of potential applications in basic and therapeutic research. This study aimed to evaluate the biological activity of totally synthesized ditetradecylacetyldiethanolaminetrimethylammonium (TMA-C2-DEA-C14) as gene

Choline deficiency associated with diethanolamine carcinogenicity.

Paul M Newberne

Toxicological sciences : an official journal of the Society of Toxicology, 67(1), 1-3 (2002-04-19)

Diethanolamine.

IARC monographs on the evaluation of carcinogenic risks to humans, 77, 349-379 (2000-12-02)

A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate.

Yusuke Obayashi et al.

The Analyst, 140(15), 5065-5073 (2015-06-24)

Digitalization of fluorogenic enzymatic assays through the use of femtoliter chamber array technology is an emerging approach to realizing highly quantitative bioassays with single-molecule sensitivity. However, only a few digital fluorogenic enzyme assays have been reported, and the variations of

Development of antibodies for determination of alkylresorcinol metabolites in human urine and elucidation of ELISA cross-reactivity.

R Wierzbicka et al.

Journal of immunological methods, 413, 12-24 (2014-07-30)

Alkylresorcinols (ARs) are amphiphilic phenolic lipids and their two main metabolites, 3-(3,5-dihydroxyphenyl)-propanoic acid (DHPPA) and 3,5-dihydroxybenzoic acid (DHBA), can be used as biomarkers of whole grain wheat and rye intake. The aim of this study was to develop antibodies against

Low ficolin-2 levels in common variable immunodeficiency patients with bronchiectasis.

M-L Metzger et al.

Clinical and experimental immunology, 179(2), 256-264 (2014-09-25)

Common variable immunodeficiency (CVID) encompasses a heterogeneous group of antibody deficiencies characterized by susceptibility to recurrent infections and sequelae, including bronchiectasis. We investigated the relevance of the lectin complement pathway in CVID patients by analysing ficolin-2 and ficolin-3 serum levels

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