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Tali H Reingewertz et al.
Biochemistry, 54(21), 3337-3347 (2015-05-13)
The leading risk factor for gastric cancer in humans is infection by Helicobacter pylori strains that express and translocate the oncoprotein CagA into host epithelial cells. Once inside host cells, CagA interacts with ASPP2, which specifically stimulates p53-mediated apoptosis and
R Ravikanth Reddy et al.
The journal of physical chemistry. B, 119(44), 14076-14085 (2015-10-09)
Interaction of small molecules with collagen has far reaching consequences in biological and industrial processes. The interaction between collagen and selected polyphenols, viz., gallic acid (GA), pyrogallol (PG), catechin (CA), and epigallocatechin gallate (EGCG), has been investigated by various solution
Michael G Mazzacavallo et al.
PloS one, 10(12), e0144300-e0144300 (2015-12-04)
Root biomass distributions have long been used to infer patterns of resource uptake. These patterns are used to understand plant growth, plant coexistence and water budgets. Root biomass, however, may be a poor indicator of resource uptake because large roots
Vincent C-C Wang et al.
The journal of physical chemistry. B, 119(43), 13690-13697 (2015-07-16)
Protein film electrochemistry has been used to investigate reactions of highly active nickel-containing carbon monoxide dehydrogenases (CODHs). When attached to a pyrolytic graphite electrode, these enzymes behave as reversible electrocatalysts, displaying CO2 reduction or CO oxidation at minimal overpotential. The
Mary Cloud B Ammons et al.
PloS one, 10(5), e0126735-e0126735 (2015-05-16)
Chronic, non-healing wounds contribute significantly to the suffering of patients with co-morbidities in the clinical population with mild to severely compromised immune systems. Normal wound healing proceeds through a well-described process. However, in chronic wounds this process seems to become
Sophie E L Bulman et al.
Journal of biomaterials applications, 29(8), 1193-1200 (2014-10-18)
As problems of antibiotic resistance increase, a continuing need for effective bioactive wound dressings is anticipated for the treatment of infected chronic wounds. Naturally derived antibacterial agents, such as Manuka honey, consist of a mixture of compounds, more than one
Xiao Jiang et al.
Biomedical microdevices, 17(1), 20-20 (2015-02-06)
Growth of the MDA-MB-231 breast cancer cell line in microfluidic channels was inhibited when culture media was delivered to the channels via microbore Tygon® tubing. Culture media incubated within this tubing also inhibited growth of these cells in conventional 96-well
Xiangfeng Meng et al.
Applied microbiology and biotechnology, 99(14), 5885-5894 (2015-01-15)
Glucansucrases are exclusively found in lactic acid bacteria and synthesize a variety of α-glucans from sucrose. They are large multidomain enzymes belonging to the CAZy family 70 of glycoside hydrolase enzymes (GH70). The crystal structure of the N-terminal truncated GTF180
Adam Magyar et al.
The journal of physical chemistry. B, 117(8), 2239-2246 (2013-01-25)
The deprotonation of naturally occurring zeaxanthin (Zea) radical cations (Zea(•+)) to form neutral radicals (#Zea(•)) and their involvement in the qE portion of nonphotochemical quenching (NPQ) was examined. The radical cations are weak acids, and readily deprotonate to a long-lived
Qingxin Li et al.
Scientific reports, 5, 13586-13586 (2015-09-01)
Erythropoiesis is regulated by the erythropoietin receptor (EpoR) binding to its ligand. The transmembrane domain (TMD) and the juxtamembrane (JM) regions of the EpoR are important for signal transduction across the cell membrane. We report a solution NMR study of
Ridwana Chowdhury et al.
Oncotarget, 6(2), 715-731 (2015-01-19)
Stromal fibroblasts become altered in response to solid cancers, to exhibit myofibroblastic characteristics, with disease promoting influence. Infiltrating mesenchymal stem cells (MSC) may contribute towards these changes, but the factors secreted by cancer cells that impact MSC differentiation are poorly
Ruthenium-Catalyzed Regioselective Deuteration of Alcohols at the ?-Carbon Position with Deuterium Oxide.
Tse SKS, et al.
Chemistry?A European Journal, 17(49), 13918-13925 (2011)
V K Sharma et al.
Soft matter, 11(34), 6755-6767 (2015-07-28)
Antimicrobial peptides are universal in all forms of life and are well known for their strong interaction with the cell membrane. This makes them a popular target for investigation of peptide-lipid interactions. Here we report the effect of melittin, an
Sonali Ghosh et al.
Journal of bacteriology, 197(5), 992-1001 (2014-12-31)
This work was undertaken to obtain information on levels of metabolism in dormant spores of Bacillus species incubated for weeks at physiological temperatures. Spores of Bacillus megaterium and Bacillus subtilis strains were harvested shortly after release from sporangia and incubated
Brandon K Tate et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 21(28), 10160-10169 (2015-06-11)
Alkoxide-bridged disilver cations react with dihydrogen to form hydride-bridged cations, releasing free alcohol. Hydrogenolysis of neutral silver fluorides affords hydride-bridged disilver cations as their bifluoride salts. These reactions proceed most efficiently when the supporting ligands are expanded N-heterocyclic carbenes (NHCs)
Vadim Cherezov et al.
Structure (London, England : 1993), 16(9), 1378-1388 (2008-09-13)
The crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support
Takhar Kasumov et al.
American journal of physiology. Heart and circulatory physiology, 304(9), H1201-H1214 (2013-03-05)
Traditional proteomics provides static assessment of protein content, but not synthetic rates. Recently, proteome dynamics with heavy water ((2)H2O) was introduced, where (2)H labels amino acids that are incorporated into proteins, and the synthesis rate of individual proteins is calculated
Madhur D Shastri et al.
PloS one, 10(5), e0126763-e0126763 (2015-05-12)
Enoxaparin, a mixture of anticoagulant and non-anticoagulant fractions, is widely used as an anticoagulant agent. However, it is also reported to possess anti-inflammatory properties. Our study indicated that enoxaparin inhibits the release of IL-6 and IL-8 from A549 pulmonary epithelial
Geoffrey W R Edwards et al.
Health physics, 107(5), 417-434 (2014-10-02)
The neutron economy and online refueling capability of heavy water moderated reactors enable them to use many different fuel types, such as low enriched uranium, plutonium mixed with uranium, or plutonium and/or U mixed with thorium, in addition to their
Tatiana Perevozchikova et al.
Journal of pharmaceutical sciences, 104(6), 1946-1959 (2015-04-08)
Adsorption of proteins to solid-fluid interfaces is often empirically found to promote formation of soluble aggregates and larger, subvisible, and visible particles, but key stages in this process are often difficult to probe directly. Aggregation mediated by adsorption to water-silicon
Jing Jing et al.
Biomacromolecules, 16(3), 715-722 (2015-01-30)
In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functionalized hyaluronic acid (HA)
Nicolas Gisch et al.
Scientific reports, 5, 16718-16718 (2015-11-19)
Members of the Mitis group of streptococci possess teichoic acids (TAs) as integral components of their cell wall that are unique among Gram-positive bacteria. Both, lipoteichoic (LTA) and wall teichoic acid, are formed by the same biosynthetic pathway, are of
Suren A Tatulian
Methods in molecular biology (Clifton, N.J.), 974, 177-218 (2013-02-14)
Fourier transform infrared (FTIR) spectroscopy is widely used in structural characterization of proteins or peptides. While the method does not have the capability of providing the precise, atomic-resolution molecular structure, it is exquisitely sensitive to conformational changes occurring in proteins
S Lotze et al.
The Journal of chemical physics, 142(21), 212436-212436 (2015-06-08)
We study the interaction between the ions methylguanidinium and trifluoroacetate dissolved in D2O and dimethylsulfoxide with linear infrared spectroscopy and femtosecond two-dimensional infrared spectroscopy. These ions constitute model systems for the side chains of arginine and glutamic and aspartic acid
Shyam K Konda et al.
Organic & biomolecular chemistry, 13(21), 5972-5982 (2015-05-02)
The binding of the anti-cancer drug pixantrone to three oligonucleotide sequences, d(TCATATGA)2, d(CCGAGAATTCCGG)2 {double bulge = DB} and the non-self complementary d(TACGATGAGTA) : d(TACCATCGTA) {single bulge = SB}, has been studied by NMR spectroscopy and molecular modelling. The upfield shifts observed for
Tom Resler et al.
Biophysical journal, 109(2), 287-297 (2015-07-23)
Channelrhodopsins (ChRs) are light-gated cation channels. After blue-light excitation, the protein undergoes a photocycle with different intermediates. Here, we have recorded transient absorbance changes of ChR2 from Chlamydomonas reinhardtii in the visible and infrared regions with nanosecond time resolution, the
Renan O Silva et al.
The Journal of pharmacy and pharmacology, 67(7), 1017-1027 (2015-03-27)
The aim of the study was to investigate the anti-inflammatory, antioxidant and antinociceptive actions of PFPe, a polysaccharide fraction isolated from the dried fruit of the Passiflora edulis. Animals were pretreated with PFPe (0.3, 1 or 3 mg/kg, i.p.) 1 h before
Plinio Innocenzi et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 16(9), 1933-1939 (2015-04-22)
The in situ observation of a sol-to-gel transition in fast evaporating systems is a challenging task and the lack of a suitable experimental design, which includes the chemistry and the analytical method, has limited the observations. We synthesise an acidic
Ryan J Smith et al.
Biophysical chemistry, 203-204, 12-21 (2015-05-23)
Poly(ethylene glycol) (PEG) is often conjugated to polyethylenimine (PEI) to provide colloidal stability to PEI-DNA polyplexes and shield charge leading to toxicity. Here, a library of nine cationic copolymers was synthesized by grafting three molecular weights (750, 2000, 5000Da) of
Thanh-Loan Lai et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 21(38), 13311-13320 (2015-08-01)
The internal functionalization of the Keplerate-type capsule Mo132 has been carried out by ligand exchange leading to the formation of glutarate and succinate containing species isolated as ammonium or dimethylammonium salts. Solution NMR analysis is consistent with asymmetric inner dicarboxylate
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