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Jessica A Bernard et al.
Frontiers in neuroanatomy, 6, 31-31 (2012-08-22)
The cerebellum plays a role in a wide variety of complex behaviors. In order to better understand the role of the cerebellum in human behavior, it is important to know how this structure interacts with cortical and other subcortical regions
Akinori Kashimura et al.
PloS one, 8(11), e78669-e78669 (2013-11-19)
Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fused to Protein A
Yue Ma et al.
The Journal of biological chemistry, 290(11), 7314-7322 (2015-02-04)
EsxA (ESAT-6), an important virulence factor of Mycobacterium tuberculosis, plays an essential role in phagosome rupture and bacterial cytosolic translocation within host macrophages. Our previous study showed that EsxA exhibits a unique membrane-interacting activity that is not found in its
Guotian Li et al.
BMC biotechnology, 18(1), 54-54 (2018-09-06)
Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel
Øyvind Strømland et al.
Biochimica et biophysica acta, 1859(5), 1029-1039 (2017-01-11)
Helix A and -C of α-lactalbumin, a loosely folded amphitropic protein, perturb lipid monolayers by the formation of amyloid pore-like structures. To investigate whether these helices are able to disrupt fully formed bilayers, we designed peptides comprised of Helix A
Rachida Abes et al.
Nucleic acids research, 36(20), 6343-6354 (2008-09-18)
Redirecting the splicing machinery through the hybridization of high affinity, RNase H- incompetent oligonucleotide analogs such as phosphoramidate morpholino oligonucleotides (PMO) might lead to important clinical applications. Chemical conjugation of PMO to arginine-rich cell penetrating peptides (CPP) such as (R-Ahx-R)(4)
Eugenio Notomista et al.
Microbial cell factories, 14, 126-126 (2015-09-05)
Pathogenic bacteria easily develop resistance to c onventional antibiotics so that even relatively new molecules are quickly losing efficacy. This strongly encourages the quest of new antimicrobials especially for the treatment of chronic infections. Cationic antimicrobial peptides (CAMPs) are small
Daniel Tello et al.
The FEBS journal, 281(11), 2558-2569 (2014-04-05)
We have used an isolated chimeric protein E1340 E2661 that includes the ectodomains of the envelope proteins of hepatitis C virus to study its interaction with model membranes. E1340 E2661 has some of the membrane destabilization properties, vesicle aggregation, lipid
Nataliya M Samoshina et al.
Pharmaceutics, 3(3), 379-405 (2011-01-01)
Incorporation of a pH-sensitive conformational switch into a lipid structure enables a drastic conformational flip upon protonation that disrupts the liposome membrane and causes rapid release of cargo specifically in areas of increased acidity. pH-sensitive liposomes containing the amphiphile (1)
Akira Tawada et al.
Glycobiology, 12(7), 421-426 (2002-07-18)
Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by
Masaya Misaki et al.
Magnetic resonance in medicine, 74(6), 1609-1620 (2014-12-24)
In order to more precisely differentiate cerebral structures in neuroimaging studies, a novel technique for enhancing the tissue contrast based on a combination of T1-weighted (T1w) and T2-weighted (T2w) MRI images was developed. The combined image (CI) was calculated as
Radda Rusinova et al.
The Journal of general physiology, 138(2), 249-270 (2011-07-27)
The thiazolidinediones (TZDs) are used in the treatment of diabetes mellitus type 2. Their canonical effects are mediated by activation of the peroxisome proliferator-activated receptor γ (PPARγ) transcription factor. In addition to effects mediated by gene activation, the TZDs cause
Sarah E Pease-Raissi et al.
Neuron, 96(2), 373-386 (2017-10-13)
Chemotherapy-induced peripheral neuropathy (CIPN) is a debilitating side effect of many cancer treatments. The hallmark of CIPN is degeneration of long axons required for transmission of sensory information; axonal degeneration causes impaired tactile sensation and persistent pain. Currently the molecular
Ashutosh Shukla et al.
Journal of virology, 88(21), 12409-12421 (2014-08-15)
Membrane-active peptides, components of capsid structural proteins, assist viruses in overcoming the host membrane barrier in the initial stages of infection. Several such peptides have been identified, and their roles in membrane fusion or disruption have been characterized through biophysical
Samir E L Andaloussi et al.
Nucleic acids research, 39(9), 3972-3987 (2011-01-20)
While small interfering RNAs (siRNAs) have been rapidly appreciated to silence genes, efficient and non-toxic vectors for primary cells and for systemic in vivo delivery are lacking. Several siRNA-delivery vehicles, including cell-penetrating peptides (CPPs), have been developed but their utility
Crystal M Darby et al.
PloS one, 8(7), e68942-e68942 (2013-08-13)
Bacterial pathogens like Mycobacterium tuberculosis (Mtb) encounter acidic microenvironments in the host and must maintain their acid-base homeostasis to survive. A genetic screen identified two Mtb strains that cannot control intrabacterial pH (pHIB) in an acidic environment; infection with either
Daniel T Cohen et al.
Cell reports, 30(10), 3229-3239 (2020-03-12)
BCL-2 family proteins converge at the mitochondrial outer membrane to regulate apoptosis and maintain the critical balance between cellular life and death. This physiologic process is essential to organism homeostasis and relies on protein-protein and protein-lipid interactions among BCL-2 family
Ann-Kristin Mossberg et al.
PloS one, 5(2), e9384-e9384 (2010-02-27)
Cell membrane interactions rely on lipid bilayer constituents and molecules inserted within the membrane, including specific receptors. HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a tumoricidal complex of partially unfolded alpha-lactalbumin (HLA) and oleic acid that is internalized
Yong Deng et al.
Carbohydrate polymers, 199, 619-627 (2018-08-26)
Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on
Sree Devi Menon et al.
The Journal of cell biology, 169(6), 909-920 (2005-06-16)
In Drosophila, myoblasts are subdivided into founders and fusion-competent myoblasts (fcm) with myotubes forming through fusion of one founder and several fcm. Duf and rolling pebbles 7 (Rols7; also known as antisocial) are expressed in founders, whereas sticks and stones
Wanchen Shao et al.
Plant methods, 16, 99-99 (2020-08-04)
Identification and characterization of key enzymes associated with cell wall biosynthesis and modification is fundamental to gain insights into cell wall dynamics. However, it is a challenge that activity assays of glycosyltransferases are very low throughput and acceptor substrates are
Ibai Artetxe et al.
Biophysical journal, 113(4), 860-868 (2017-08-24)
Ceramide is a sphingolipid involved in several cellular processes, including apoptosis. It has been proposed that ceramide forms large and stable channels in the mitochondrial outer membrane that induce cell death through direct release of cytochrome c. However, this mechanism
Jonathan Zerweck et al.
Scientific reports, 7(1), 13153-13153 (2017-10-17)
PGLa and magainin 2 (MAG2) are amphiphilic α-helical membranolytic peptides from frog skin with known synergistic antimicrobial activity. By systematically mutating residues in the two peptides it was possible to identify the ones crucial for the synergy, as monitored by
Julio Acosta-Cabronero et al.
PloS one, 8(11), e81093-e81093 (2013-11-28)
This study explores the magnetostatic properties of the Alzheimer's disease brain using a recently proposed, magnetic resonance imaging, postprocessed contrast mechanism. Quantitative susceptibility mapping (QSM) has the potential to monitor in vivo iron levels by reconstructing magnetic susceptibility sources from
Vijay K Venkatraman et al.
Frontiers in aging neuroscience, 12, 115-115 (2020-05-16)
White matter (WM) microstructure is a sensitive marker to distinguish individuals at risk of Alzheimer's disease. The association of objective physical fitness (PF) measures and WM microstructure has not been explored and mixed results reported with physical activity (PA). Longitudinal
Lisa Byrge et al.
Human brain mapping, 41(9), 2249-2262 (2020-03-10)
Despite enthusiasm about the potential for using fMRI-based functional connectomes in the development of biomarkers for autism spectrum disorder (ASD), the literature is full of negative findings-failures to distinguish ASD functional connectomes from those of typically developing controls (TD)-and positive
Carmen L Delgado et al.
Biochimica et biophysica acta, 1848(4), 895-906 (2015-01-03)
In a previous study, it was shown that purified preS domains of hepatitis B virus (HBV) could interact with acidic phospholipid vesicles and induce aggregation, lipid mixing and leakage of internal contents which could be indicative of their involvement in
Byung-Ju Jin et al.
Lab on a chip, 17(5), 887-895 (2017-02-12)
Water transport across epithelial monolayers is of central importance in mammalian fluid homeostasis, and epithelial aquaporin (AQP) water channels are potential drug targets. Current methods to measure transepithelial water permeability based on indicator dilution have limited accuracy and can require
Satoshi Wakita et al.
FEBS letters, 591(20), 3310-3318 (2017-08-24)
Mouse acidic mammalian chitinase (AMCase) degrades chitin with highest efficiency at pH 2.0 and is active up to pH 8.0. Here, we report that mouse AMCase also exhibits transglycosylation activity under neutral conditions. We incubated natural and artificial chitin substrates
Nico Lämmerhardt et al.
Carbohydrate research, 489, 107933-107933 (2020-02-13)
Various (semi)-synthetic non-digestible glucans (NDG) (resistant (malto)dextrin, isomaltooligosaccharide, soluble corn fiber, polydextrose) were analyzed by methylation analysis, 1H NMR spectroscopy, ESI-MSn, TLC, and capillary electrophoresis (CE/UV). Partly complementary and partly corroborative results were obtained by these methods yielding a comprehensive
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