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G005707

HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)
mobile phase: [A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; (70:30, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μL
sample: 20 μg/mL in 80:12:8, water: acetonitrile: dimethylsulfoxide

suitability

application for HPLC

application(s)

industrial hygiene

Analysis Note

This application demonstrates the suitability of Ascentis Cyano for the efficient separation of isocyanate derivatives.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analytical column

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