Smart SPME fiber assemblies combine innovative SPME devices with Smart technology for seamless sample preparation. Each Smart fiber assembly, specifically designed to be compatible with the PAL 3, Series II Smart autosampler, incorporates a SPME fiber within a holder and a programmable computer chip coded with fiber chemistry type, dimensions, and lot number. The Smart chip provides traceability by recording fiber stroke count, injection and conditioning durations, usage dates, and expiration date. The one piece fiber/holder design makes the Smart SPME assembly easier to handle and install than traditional SPME. In addition, compatibility with the SPME tool on the PAL 3 autosampler allows for more efficient method development and completely automated sample preparation, thus increasing overall productivity.