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Anti-MYH1 Antibody, clone 1E15 , ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

MyHC-2x, MyHC-IIx/d, Myosin heavy chain, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin-1, skeletal muscle adult 1

biological source

rabbit (recombinant)


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


1E15, recombinant monoclonal

product line

ZooMAb® learn more



mol wt

calculated mol wt 223.15 kDa
observed mol wt ~225

species reactivity

mouse, human


antibody small pack of 25 μL

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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable



UniProt accession no.

greener alternative category

shipped in


storage temp.


Gene Information

human ... MYH1(4619)

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 1E15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myosin Heavy Chain 1. it targets an epitope within 18 amino acids from the N-terminal region.


KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Myosin Heavy Chain 1.


Anti-MYH1, clone 1E15 ZooMAb, Cat. No. ZRB1214, is a highly specific recombinant rabbit monoclonal antibody that detects Myosin heavy chain 1 (MYH1) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin) and, Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MYH1 in C2C12 cell lysate.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MYH1 in human skeletal muscle and mouse skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

Target description

Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain, skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and non-muscle cells. These isoforms show expression that is spatially and temporally regulated during development. Myosin-1 contains an N-terminal SH3-like domain (aa 33-82), a myosin motor domain (aa 86-785), and an IQ domain (aa 788-817). It also contains 2 actin binding regions (aa 662-684 and 764-778). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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