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T3809

Sigma-Aldrich

TRI Reagent®

BD, For processing whole blood, plasma, or serum.

Synonym(s):
TRI Reagent RNA Isolation Reagent
MDL number:
NACRES:
NA.52

Quality Level

usage

0.75 mL sufficient for 0.25 mL blood derivatives

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General description

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.
TRI Reagent® is a monophasic solution of phenol and guanidinium isothiocyanate.

Application

The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.
TRI Reagent® has been used for the isolation of RNA.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Packaging

25, 100, 200 mL in glass bottle

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Certificate of Analysis

Certificate of Origin

A novel CLCN5 mutation in a Chinese boy with Dent's disease.
Ji LN
World Journal of Pediatrics : WJP, 10(3), 275-277 (2014)
Circulating ribonucleic acids and metabolic stress parameters may reflect progression of autoimmune or inflammatory conditions in juvenile type 1 diabetes.
Kocic G
TheScientificWorldJournal, 11, 1496-1508 (2011)
Purification of RNA using TRIzol (TRI reagent)
Rio D, et al.
Cold Spring Harbor Protocols, 2010(6), pdb-prot5439 (2010)
Xuan Li et al.
PLoS genetics, 7(6), e1002119-e1002119 (2011-06-23)
SKN-1, the Caenorhabditis elegans Nrf1/2/3 ortholog, promotes both oxidative stress resistance and longevity. SKN-1 responds to oxidative stress by upregulating genes that detoxify and defend against free radicals and other reactive molecules, a SKN-1/Nrf function that is both well-known and
Marian Caikovski et al.
PLoS genetics, 4(8), e1000165-e1000165 (2008-08-30)
Arabidopsis MOM1 is required for the heritable maintenance of transcriptional gene silencing (TGS). Unlike many other silencing factors, depletion of MOM1 evokes transcription at selected loci without major changes in DNA methylation or histone modification. These loci retain unusual, bivalent

Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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