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SAB1411733

Sigma-Aldrich

Monoclonal Anti-HA antibody produced in mouse

clone 1B8, purified immunoglobulin, buffered aqueous solution

NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B8, monoclonal

form

buffered aqueous solution

mol wt

antigen 75 kDa

species reactivity

human

technique(s)

indirect ELISA: suitable

isotype

IgG1κ

NCBI accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... HA(3654620)

General description

Influenza virus hemagglutinin (HA) precursor is encoded by the gene mapped on segment 4. The encoded protein is a 76 kDa surface glycoprotein and exists as trimers on the envelope.

Immunogen

Recombinant Fc fusion protein corresponding to amino acids 17-531 of virus HA (Influenza A virus, A/Viet Nam/3062/2004 (H5N1))

Sequence
DQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKTHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRERRRKKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYSEEARLKREEISGVKLESIGIYQ

Application

Monoclonal Anti-HA antibody produced in mouse has been used in immunoprecipitation.

Biochem/physiol Actions

Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype plays a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic.

Physical form

Solution in phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Ashraf Metwally et al.
International journal of molecular sciences, 18(4), 1-18 (2017-04-05)
Successful immunization against avian influenza virus (AIV) requires eliciting an adequate polyclonal response to AIV hemagglutinin (HA) subunit 1 (HA1) epitopes. Outbreaks of highly-pathogenic (HP) AIV subtype H5N1 can occur in vaccinated flocks in many endemic areas. Protection against emerging
The role of influenza A virus hemagglutinin residues 226 and 228 in receptor specificity and host range restriction.
Vines A, et.al.
Journal of Virology, 72(9), 7626-7631 (1998)
A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity.
Glaser L
Journal of Virology, 79, 11533-11536 (2005)
20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis.
Liu X, et.al.
The Journal of Biological Chemistry, 290(45), 27370-27383 (2015)
Visualization of Alternative Functional Configurations of Influenza Virus Hemagglutinin Facilitates Rapid Selection of Complementing Vaccines in Emergency Situations.
Metwally A and Yousif A
International Journal of Molecular Sciences, 18(4) (2017)

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