RealSeq® is a novel method for preparing small-RNA sequencing libraries that reduces incorporation bias in Next-Generation-Sequencing (NGS). Most bias stems from sequence-dependent variability in the enzymatic ligation reactions that attach the two adapters to the 3′ and 5′ ends of the miRNAs/small RNAs during preparation of sequencing libraries. By using a novel single adapter and circularization, RealSeq® greatly reduces library preparation bias. This technology solves the problem of commonly used sequencing library preparations that lead to underdetection of many miRNAs.
RealSeq®-AC is a small RNA library preparation kit compatible with Illumina Next Generation Sequencing (NGS) technology. RealSeq®-AC is optimized for RNA input between 1ng and 1000ng of total RNA from tissue or cells. This kit is for small RNA and miRNA library preparation for NGS. RealSeq-AC is highly efficient, detecting more miRNAs in total RNA samples.
RealSeq®-AC has been used for the construction of sRNA libraries from Rosa chinensis, Aquilegia coerulea, Triticum aestivum, and Hordeum vulgare.
RealSeq® accurately quantifies small RNA sequenced on the Illumina Platform. RealSeq®-AC uses ligation of a single adapter producing small RNA libraries with more miRNAs compared to library prep kits using two adapters.
Features and Benefits
- Significantly reduces sequencing bias in small-RNA sequencing
- Accurately quantifies biologically relevant small RNAs
- Allows for more efficient discovery of novel small RNAs
This kit is for 12 libraries. This product is comprised of the following:
Dimer Removal Agent
Rnase Free Water
Reverse Index Primers*
Adapter Dilution Buffer
* Index Primers 1-12 are included in REALSEQAC-1KT (500-00012)
See experimental protocol for more information.
Storage and Stability
The three components should be stored at the specified temperature upon arrival of the Kit.
- RealSeq®-AC (BOX 1) at −20 °C
- RealSeq®-AC (BOX 2) at −20 °C
- RealSeq®-AC (Tube with Beads 3) at 2-8 °C
RealSeq is a registered trademark of SomaGenics, Inc.