MilliporeSigma
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RAB0275

Sigma-Aldrich

Mouse IL-1 β ELISA Kit

for cell and tissue lysates

NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il1b(16176)

General description

The Mouse IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 β cell lysate and tissue lysate.

Immunogen

Recombinant Mouse IL-1 β

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse IL-1 β ELISA Kit has been used to measure the concentration of interleukin 1 (IL)1-β in serum[73] and bronchoalveolar lavage fluid (BALF) sample.[72]

Biochem/physiol Actions

Interleukin 1 β (IL-1β) stimulates the production of endogenous 92 kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1).[96] It is a proinflammatory cytokine that induces various acute and chronic inflammation. Therefore, IL-1β can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling.[03] IL-1β brings neutrophil and macrophage to the site of infection.[06] Over expression of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint.[68] Mutations in IL-1β gene leads to renal manifestations and renal sequelae in Henoch-Schonlein purpura (HSP).[09]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABLYSIS12X Cell Lysis Buffer (Item J)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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