P9029

o-Phenylenediamine

Peroxidase substrate, ≥98.0%, powder

• Synonym(s): 1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD
• Empirical Formula (Hill Notation): C₆H₈N₂
• CAS Number: 95-54-5
• Molecular Weight: 108.14
• Beilstein: 606074
• EC Number: 202-430-6
• MDL number: MFCD00007721
• PubChem Substance ID: 24899004
• NACRES: NA.83

Properties

• vapor density: 3.7 (vs air)
• Quality Level: 200
• vapor pressure: 0.01 mmHg ( 25 °C)
• assay: ≥98.0%
• form: powder
• color: white to off-white
• bp: 256-258 °C
• mp: 98-102 °C
• solubility: ethanol: 50 mg/mL, clear, colorless to faintly yellow
• fluorescence: λ_ex 337 nm; λ_em 417 nm (Pyruvic acid derivative)
• storage temp.: 2-8°C
• SMILES string: Nc1ccccc1N
• InChI: 1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
• InChI key: GEYOCULIXLDCMW-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H₂SO₄ and read at 492 nm.

Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification.[06] It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.[07][08]

Packaging

50, 100 g in glass bottle

Caution

May darken in storage.

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 - H312 + H332 - H317 - H319 - H341 - H351 - H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 276.8 °F - closed cup
• Flash Point(C): 136 °C - closed cup