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P3476

Millipore

Protein A–Agarose Fast Flow

50%, aqueous suspension

Synonym(s):
Protein A resin
MDL number:
NACRES:
NA.56

biological source

protein A from Staphylococcus aureus

Quality Level

description

protein A leaching <= 5 ng/mL

form

aqueous suspension

concentration

50%

matrix

6% Fast-flow beaded agarose

capacity

37-43 mg/mL binding capacity (IgG)

storage temp.

2-8°C

Application

High capacity, low leakage, stable affinity medium for the purification of monoclonal and polyclonal antibodies. Ideal for rapid capture of immunoglobulins from cell culture.
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Packaging

1, 5 mL in glass bottle

Physical form

Suspension containing 0.01% thimerosal

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Phosphoprotein Inhibitors of Protein Phosphatase-1
Eto, M., et al.
Methods in Enzymology, 366, 241-260 (2003)
Nsi1 plays a significant role in the silencing of ribosomal DNA in <I>Saccharomyces cerevisiae</I>.
Ha, C.W., et al.
Nucleic Acids Research (2012)
Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) for Studying Dynamics of Protein Abundance and Posttranslational Modifications.
Amanchy, R., et al.
Science Signaling, 2005(267), pI2-pI2 (2005)
Boris Gorbatyuk et al.
Molecular microbiology, 55(4), 1233-1245 (2005-02-03)
DnaA protein binds bacterial replication origins and it initiates chromosome replication. The Caulobacter crescentus DnaA also initiates chromosome replication and the C. crescentus response regulator CtrA represses chromosome replication. CtrA proteolysis by ClpXP helps restrict chromosome replication to the dividing
Y Shimazaki et al.
Journal of biochemical and biophysical methods, 37(1-2), 1-4 (1998-11-24)
Protein spots, such as immunoglobulin G (IgG), A (IgA) or alpha 2-macroglobulin, on the patterns of non-denaturing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) were selectively removed by the application of protein A or antibody-conjugated protein A agarose to the first-dimension

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