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Key Documents

N9414

Sigma-Aldrich

Anti-NUCB2 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Calnuc, Anti-NEFA, Anti-Nef, Anti-Nesfatin, Anti-Nucleobindin 2, Anti-p54

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~54 kDa

species reactivity

rat, mouse

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using Rat2 cells
western blot: 1.5-3.0 μg/mL using Rat2 cell lysates
western blot: 2-4 μg/mL using mouse testis extracts (S1 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

NUCB2 (N-terminal) (nucleobindin 2, also known as NEFA, p54) belongs to the nucleobindin family of EF-hand Ca2+-binding proteins.
Nucleobindin 2 (NUCB2), contain multiple functional domains, including a Leu/Ile-rich region, a putative nuclear localization signal, a DNA binding domain, two Ca2+ -binding EF-hand motifs, and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. Human NUCB2 has high homology (62% sequence identity) with human NUCB1, an additional member of the nucleobindin family, although they are encoded by two separate genes.

Application

Anti-NUCB2 (N-terminal) antibody produced in rabbit has been used in:
  • immunofluorescence
  • western blotting
  • immunohistochemistry

Biochem/physiol Actions

Nucleobindin 2 (NUCB2) is implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding, and tumor necrosis factor (TNF)-receptor-1 (TNFR1) shedding. NUCB2 has been shown to interact with both necdin and endoplasmic reticulum aminopeptidase 1 (ARTS-1), both through the EF-hand motif. NUCB2-ARTS-1 complex is required for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been recently identified as a novel satiety molecule in the hypothalamus, associated with melanocortin signaling in the central nervous system. NUCB2 and its N-terminal 82-amino acid cleavage fragment, termed nesfatin-1, have been shown to induce suppression of food-intake in rats.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paraventricular NUCB2/nesfatin-1 supports oxytocin and vasopressin neurons to control feeding behavior and fluid balance in male mice
Nakata M, et al.
Endocrinology, 157(6), 2322-2332 (2016)
Possible involvement of hypothalamic nucleobindin-2 in hyperphagic feeding in Tsumura Suzuki obese diabetes mice
Miyata S, et al.
Biological & Pharmaceutical Bulletin, 35(10), 1784-1793 (2012)
Nesfatin-1 and its effects on different systems
Ayada, C and Toru,
Hippokratia, 19(1), 4-4 (2015)
The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm
Taniguchi N, et al.
The Journal of Biological Chemistry, 275(41), 31674-31681 (2000)
Masaru Shimizu et al.
Aging, 12(23), 24134-24140 (2020-12-16)
NUCB2/nesfatin-1 was originally discovered as an anorexigenic peptide. However, recent studies revealed various additional functions including the regulation of inflammation. However, there are no studies that investigated the involvement of NUCB2/nesfatin-1 in neuroinflammatory diseases. Here, we aimed to investigate the

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