All Photos(2)

N0766

Sigma-Aldrich

Nile Blue A

certified by the Biological Stain Commission

Synonym(s):
Basic Blue 12, Nile blue sulfate
Linear Formula:
2C20H20N3O · SO4
CAS Number:
Molecular Weight:
732.85
Colour Index Number:
51180
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

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Application

Nile Blue A has been used for the staining of eggs before transferring them to the hosts. It has been used for the staining of cells.

Packaging

5, 25 g in glass bottle

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

Suitability

Certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Translation of incenp during oocyte maturation is required for embryonic development in Xenopus laevis.
Leblond GG
Biology of Reproduction, 86, 1-1 (2012)
The roles of maternal alpha-catenin and plakoglobin in the early Xenopus embryo.
Kofron M
Development, 124, 1553-1553 (1997)
Electrospun bioactive mats enriched with Ca-polyphosphate/retinol nanospheres as potential wound dressing.
Muller WEG
Biochemistry and Biophysics Reports, 3, 150-160 (2015)
J Godoy-Navajas et al.
Analytica chimica acta, 701(2), 194-199 (2011-08-02)
A long-wavelength fluoroimmunoassay for the determination of soy protein is reported for the first time using a conjugate composed of anti-soy protein antibodies bound to nile blue-doped silica nanoparticles (NPs). These NPs have been synthesized by a reverse-micelle microemulsion method
J Godoy-Navajas et al.
Journal of agricultural and food chemistry, 59(6), 2235-2240 (2011-03-04)
A method for the determination of the antioxidant capacity using long-wavelength fluorescence measurements is described for the first time. This method is a modification of the conventional oxygen radical absorbance capacity (ORAC) method that uses fluorescein or phycoerythrin and the

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