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MAK209

Sigma-Aldrich

Granzyme B Inhibitor Screening Kit

Sufficient for 100 Fluorometric tests

Synonym(s):
GZMB Inhibitor Screening Kit
EC Number:
NACRES:
NA.25

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)

Related Categories

General description

Granzyme B (GZMB; EC 3.4.21.79) is a serine protease stored in the secretory granules of leukocytes. It induces cytotoxic T lymphocyte (CTL)-mediated apoptosis by the activation of caspases and the degradation of structural proteins of the target cells. Granzyme B has an important role in CTL-mediated immune responses and has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, coronary syndrome, and chronic angina pectoris.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the screening of inhibitors of Granzyme B.

Principle

The Granzyme B Inhibitor Screening Kit is a simple and high-throughput assay for screening potential inhibitors of granzyme B. Granzyme B activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate to yield AFC, a fluorescent product (λex = 380/ λem = 500 nm), proportional to the enzymatic activity present. The substrate includes the recognition sequence for granzyme B.

Kit Components Only

Product No.
Description

  • Granzyme B Assay Buffer

  • Granzyme B Substrate

  • Granzyme B Enzyme

  • Inhibitor Control, Ac-IEPD-CHO

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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