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M0275

Sigma-Aldrich

Minimum Essential Medium Eagle

10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
MEM

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
HEPES: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Application

Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.
Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Karl Norris et al.
Micromachines, 10(1) (2019-01-24)
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial...
Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure
Meder R, et al.
Osteoarthritis and Cartilage, 14(9), 875-881 (2006)
Collagen-lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation
Vandrovcova M, et al.
Journal of Biomedical Materials Research Part A, 103(2), 525-533 (2015)
Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs
Gurkan UA, et al.
Journal of Biomedical Materials Research Part A, 94(4), 1070-1079 (2010)
A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine
Drzewiecki KE, et al.
Technology, 5(04), 185-195 (2017)

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