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L4021

Sigma-Aldrich

Prolactin human

recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):
hPRL, LTH, Lactogenic Hormone, Luteotropic Hormone, PRL
CAS Number:
MDL number:

recombinant

expressed in E. coli

Quality Level

description

200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227).

product line

BioReagent

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.030-0.300 ng/mL EC50

mol wt

predicted mol wt ~24 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: 0.5000 mL, clear, colorless

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... PRL(5617)

Application

Use in cell culture applications to study prolactin regulation of cell signaling and gene expression.

Biochem/physiol Actions

Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes, and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

Other Notes

Contains an N-terminal methionine not present in the natural product.

Physical form

Lyophilized from a solution in 10 mM phosphate and 50 mM sodium chloride, pH 8.0.

Reconstitution

It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Analysis Note

The biological activity is assayed by proliferation of the rat lymphoma cell line, Nb2-11.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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