GE29-0486-65

HiTrap^™ Protein L

Cytiva 29-0486-65, pack of 1 mL

• Synonym(s): Protein L chromatography
• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: recombinant protein L (E. coli), mammalian free
• packaging: pack of 1 mL
• manufacturer/tradename: Cytiva 29-0486-65
• availability: not available in North America
• storage condition: (20% Ehtanol)
• parameter: 5 bar
• column I.D.: 7 mm
• matrix: rigid, highly cross-linked agarose
• avg. part. size: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• working range: 2-10(pH interval where the medium can be operated without significant change in function.)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Description

General description

HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.

Application

Affinity chromatoghraphy; capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs.

Features and Benefits

• Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).
• Enables a platform approach to antibody fragment purification.
• High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
• Convenient for process development, as well as small-scale purification.
• HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

HisTrap is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids