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G8255

Sigma-Aldrich

Glutamic-Pyruvic Transaminase from porcine heart

lyophilized powder, ≥75 units/mg protein

Synonym(s):
L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT
CAS Number:
Enzyme Commission number:
Beilstein:
3562140
EC Number:
MDL number:
NACRES:
NA.54

biological source

Porcine heart

Quality Level

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, 75-95%

foreign activity

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.1%

storage temp.

−20°C

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General description

Glutamic-pyruvic transaminase (GPT) is localized majorly in the liver.[22]

Application

Glutamic-Pyruvic Transaminase from porcine heart has been used:
  • in the transamination assay with a racemic mixture of synthetic 3-methyl-2-oxoglutarate
[29]
  • to remove glutamate from the cell culture medium used for culturing human embryonic kidney (HEK293) cells
  • [08]
  • in the lactate-dehydrogenase (LD)-coupled alanine transaminase (ALT) reaction kinetic studies
  • [30]

    Packaging

    200 units in glass bottle
    1000, 2000 units in poly bottle

    Biochem/physiol Actions

    Glutamic-pyruvic transaminase (GPT) mediates the transfer of the amino group to α-ketoglutarate from l-alanine.[22] It plays a key role in gluconeogenesis and amino acid metabolism. The role of GPT in various kinds of tumors has been reported.[58]
    Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

    Unit Definition

    One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

    Physical form

    Lyophilized powder containing citrate and acetate

    Analysis Note

    Protein determined by biuret

    Pictograms

    Health hazard

    Signal Word

    Danger

    Hazard Statements

    Precautionary Statements

    Hazard Classifications

    Resp. Sens. 1

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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    Technical Information

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