Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.
Galactosyltransferase from bovine milk has been used:
- in the generation of hyper-galactosylated variant of IgG1
- in β -1,4-galactosyltransferase assay rice root cell extracts
- in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers
Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.
5, 10 units in serum bottle
25 units in glass bottle
Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states.
This is probably the same enzyme as N-acetyllactosamine synthetase, EC 184.108.40.206 and EC 220.127.116.11.
One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)
Lyophilized powder containing Tris, EDTA, and (NH4)2SO4