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FNCAS9TRACR

Sigma-Aldrich

SygRNA™ FnCas9 Synthetic tracrRNA

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NACRES:
NA.51

packaging

pkg of 1 vial

shipped in

ambient

storage temp.

−20°C

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TRACRRNA05NTRACRRNAMOD8.55014
SygRNA™ FnCas9 Synthetic tracrRNA

Sigma-Aldrich

FNCAS9TRACR

SygRNA™ FnCas9 Synthetic tracrRNA

SygRNA™ Cas9 Synthetic tracrRNA SygRNA™ SpCas9 tracrRNA

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TRACRRNA05N

SygRNA™ Cas9 Synthetic tracrRNA

SygRNA™ Cas9 Synthetic Modified tracrRNA

Sigma-Aldrich

TRACRRNAMOD

SygRNA™ Cas9 Synthetic Modified tracrRNA

NovaSyn®TG amino resin (130 µm) Novabiochem®

Sigma-Aldrich

8.55014

NovaSyn®TG amino resin (130 µm)

packaging

pkg of 1 vial

packaging

pkg of 5 nmol × Tube (HPLC)

packaging

pkg of 1 vial

packaging

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-30°C

General description

The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA

Application

Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology

Features and Benefits

HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion.

Physical form

Dry

Preparation Note

Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less.

Legal Information

CRISPR Use License Agreement
SygRNA is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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