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FG0325
Minimum Essential Medium Eagle
With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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Quality Level
sterility
sterile-filtered
sterile
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
components
sodium pyruvate: no
HEPES: no
L-glutamine: no
glucose: yes
stable glutamine: yes
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Application
Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.
Other Notes
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
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Certificate of Origin
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