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EDEGLY

Sigma-Aldrich

Enzymatic Protein Deglycosylation Kit

NACRES:
NA.32

Quality Level

shipped in

wet ice

storage temp.

2-8°C

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Description

  • PNGase F 1 mL/vial

  • O-Glycosidase 20 μL

  • α-2(3,6,8,9)-Neuraminidase 20 μL

  • Fetuin Control .5 mg

  • 5× Reaction Buffer .2 mL

  • Denaturation Solution .1 mL

  • Triton X-100 .1 mL

  • β-1→4-Galactosidase, positionally specific 20 μL

  • β-N-Acetylglucosaminidase 20 μL

See All (9)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Axel T Lehrer et al.
Vaccine, 36(22), 3090-3100 (2017-02-22)
Infections with filoviruses in humans are highly virulent, causing hemorrhagic fevers which result in up to 90% mortality. In addition to natural infections, the ability to use these viruses as bioterrorist weapons is of significant concern. Currently, there are no...
Identification of histoplasma-specific peptides in human urine
Crockett DK, et al.
International Journal of Peptides, 2012 (2012)
Scabies mite peritrophins are potential targets of human host innate immunity
Mika A, et al.
PLoS Neglected Tropical Diseases, 5(9), e1331-e1331 (2011)
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families...
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)

Articles

O-Linked Glycan Strategies

There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

N-Linked Glycan Strategies

N-Linked Glycan Strategies; Sigma-Aldrich.com

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