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E0778

Sigma-Aldrich

Ephrin-B2/Fc Chimera from mouse

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):
Htk-L, ELF-2, LERK-5, NLERK-1
MDL number:
NACRES:
NA.32

Quality Level

biological source

mouse

recombinant

expressed in NSO cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer calculated mol wt 49.6 kDa
60-65 kDa by SDS-PAGE (reducing)

packaging

pkg of 200 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Efnb2(13642)

Biochem/physiol Actions

Member of ephrin ligand family that binds Eph receptor tyrosine kinases (EphA4, EphB1, EphB2, EphB3, and EphB4). Involved in pattern formation and morphogenesis. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.

Other Notes

Extracellular domain of mouse ephrin-B2 (amino acids 1-227) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to bind immobilized recombinant mouse EphB2/Fc in ELISA.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Quotes and Ordering

Takahiro Sawada et al.
PloS one, 12(7), e0180785-e0180785 (2017-07-08)
We previously reported that EphA4, a member of the Eph family of receptor tyrosine kinases, is an important modulator of growth hormone (GH) signaling, leading to augmented synthesis of insulin-like growth factor 1 (IGF1) for postnatal body growth. In the

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