D5546

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
DME, DMEM
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
sodium pyruvate: yes
glucose: low
NaHCO3: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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General description

This modification has been shown to improve the stability of the medium.

Reconstitution

Supplement with 0.584 g/L glutamine.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Phuc Van Pham et al.
Stem cell research & therapy, 4(4), 91-91 (2013-08-07)
Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with...
Pratap C Naha et al.
Toxicology and applied pharmacology, 246(1-2), 91-99 (2010-04-28)
The immunotoxicity of three generations of polyamidoamine (PAMAM) dendrimers (G-4, G-5 and G-6) was evaluated in mouse macrophage cells in vitro. Using the Alamar blue and MTT assays, a generation dependent cytotoxicity of the PAMAM dendrimers was found whereby G-6...
Scott A Hinger et al.
Cell reports, 25(3), 715-725 (2018-10-18)
The regulation and functional roles of secreted coding and long noncoding RNAs (lncRNAs; >200 nt) are largely unknown. We previously showed that mutant KRAS colorectal cancer (CRC) cells release extracellular vesicles (EVs) containing distinct proteomes, microRNAs (miRNAs), and circular RNAs....
Jun Miyaguchi et al.
The Tohoku journal of experimental medicine, 245(4), 269-275 (2018-08-31)
Bacteria have been used for more than a century to treat solid tumors. Because solid tumors generate an anaerobic environment, we evaluated the anti-tumor effect of the obligate anaerobe strain KK378, derived from Lactobacillus casei (L. casei), using mice bearing...
Lei Xi Chen et al.
The Laryngoscope, 129(9), 2139-2146 (2019-03-19)
Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure...

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