CS0390

Mitochondria Staining Kit

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

• Synonym(s): JC-1 dye
• NACRES: NA.32

Properties

• Quality Level: 200
• usage: kit sufficient for 200 tests (of 1 mL cell suspensions); kit sufficient for 40 tests (of 5 mL cell suspensions)
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• technique(s): flow cytometry: suitable; protein staining: suitable
• fluorescence: λ_ex 490 nm; λ_em 530 nm (green) (JC-1 monomers); λ_ex 525 nm; λ_em 590 nm (red) (JC-1 aggregates)
• application(s): cell analysis; detection
• detection method: fluorometric
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

Application

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

Features and Benefits

Kit offers:

• A fast, simple, and convenient method for staining and assaying mitochondria intactness
• Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
• Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
• The shift in fluorescence is clearly detectable in both green and red channels
• Suitable for adherent cells as well as cells in suspension
• Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
• The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry

Kit Components Only

• DMSO 1 mL
• JC Staining Buffer 5X 120 mL
• JC-1 1 mg
• Valinomycin Ready Made .1 mL

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable