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Key Documents

Hepa-E1

99072812

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About This Item

UNSPSC Code:
41106514

biological source

eel liver

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Eel liver

Cell Line Description

The hepatocyte cell line Hepa-E1 was derived from water eel. The cells show no contact inhibition and have been reported to show apoptosis in the presence of actinomycin D, humic acid or TGF-β 1. Normal culture conditions are at 26-28°C, but maintenance at 4°C for up to 400 days was described as well.

Culture Medium

E-RDF (Kyokuto Pharmaceuticals)+ 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% trypsin or trypsin/EDTA 26-28°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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