Cell Line Origin
Human B-type EBNA negative lymphoma
Cell Line Description
The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.
B lymphoma studies, cancer treatments
RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Maintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°C
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