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BHL-89

98070109

biological source

human lymph (axillary)

growth mode

Suspension

karyotype

Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+

morphology

Lymphoblastoid

products

Not specified

receptors

sIgM,CD22,BB1,HLA-ABC,GnRH

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human B-type EBNA negative lymphoma

Cell Line Description

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

Application

B lymphoma studies, cancer treatments

Culture Medium

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO2; 37°C

Other Notes

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Certificate of Analysis

Certificate of Origin

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