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Nb2-11 Cell Line from rat

Thymus/lymph node, lymphoblast morphology, 97041101

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eCl@ss:
32190102

biological source

rat lymph

growth mode

Suspension

karyotype

42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?)

morphology

Lymphoblast

products

Not specified

receptors

Modified prolactin receptor (the Nb2 form)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat lymphoma

Cell Line Description

Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.

Application

1) in vitro bioassay of lactogenic hormones.
2) Studies of the mechanisms of lactogen induced mitogenesis.
3) Studies into the action of vinca alkaloids.
4) Studies of tumour progression.

Culture Medium

Fischer′s medium + 10% Fotal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Sodium Bicarbonate + 0.05 mM 2-Mercaptoethanol (2ME) + 2 mM Glutamine.

Subculture Routine

At resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4 x 1,000 and 12 x 1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9 x 100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  6. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  7. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

  8. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

  9. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  10. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

  11. How to freeze down Cell Line cells to make a cell stock?

    A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

  12. Are the Cell Line cells tested for viral pathogens?

    HPA Cultures does not perform any viral testing on the cell lines.

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