Cell Line Origin
Human Caucasian T Lymphocyte, mycosis fungoides.
Cell Line Description
The cell line My-La, CD8+, also known as My-La, marker chromosome was established from a plaque biopsy of an 82 year old Caucasian patient with mycosis fungoides stage II. Together with My-La, CD8+ a second cell line has been established (My-La, CD4+ Sigma Catalogue number. 95051032) by including IL-2 and IL-4 in the culture medium. My-La, CD8+ shows characteristic chromosome markers, express ß-13 of the T-cell receptor and are double homozygous for HLA-A1.B8. Phenotypically a weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / ß. There has been no evidence of HTLV-I infection in the cell line and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. My-La, CD8+ cells show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.
Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.
RPMI 1640 + 2mM Glutamine + 100 u/ml IL-2 + 10% human AB seum
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Split only 1:2 as maximum dilution: freeze in growth medium plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures.
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