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My-La CD4+

95051032

biological source

human lymph

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian CD4+ T Lymphocyte, mycosis fungoides

Cell Line Description

Two different continuous cell lines were established from a plaque biopsy of an 82-year old male with mycosis fungoides stage II by inclusion of IL-2 and IL-4 in the culture medium: My-La, CD4+, (referred to in the literature as My-La, marker chromosome) and My-La, CD8+ (referred to in the literature as My-La 46,XY-18+i(18q) (Sigma Catalogue number. 95051033). My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La CD8+ on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A1.B8. My-La CD8+ expresses β-13 of the T cell receptor and phenotypically weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / ß. My-La CD4+ expresses β-18 of the T cell receptor plus staining for CD2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 α/ ß. There has been no evidence of HTLV-I infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.

Application

Study of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 8,11
D5S818: 9,12
D7S820: 10,11
THO1: 6
TPOX: 8,12
vWA: 17

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Split only 1:2 as maximum dilution: freeze in 45% growth medium, 45% human AB serum plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures. Population doubling time 20-24 hours.

Other Notes

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Certificate of Analysis

Certificate of Origin

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