All Photos(1)

DOK

94122104

biological source

human mouth

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial

products

p53

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian dysplastic oral keratinocyte

Cell Line Description

The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.

Application

Study of oral keratinocytes, multi-stage oral carcinogenesis and carcinogens

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 9,12
D5S818: 12
D7S820: 11,13
THO1: 6,8
TPOX: 10
vWA: 14,18

Culture Medium

DMEM + 2mM Glutamine + 5μg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

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