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HGC-27

94042256

biological source

human lymph

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Mucin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human gastric carcinoma

Cell Line Description

Undifferentiated, mucin producing.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 10,11
D5S818: 12
D7S820: 11,12,13
THO1: 9
TPOX: 8
vWA: 14

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

Certificate of Origin

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