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product name
SK LU 1, 93120835
biological source
human lung
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian lung adenocarcinoma
Cell Line Description
Derived from a 64 year old Caucasian female with lung adenocarcinoma (grade III). The line is tumourigenic in immunotolerant rats. HLA = Aw24, w32, w41, B27
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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