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COLO 206F

93052620

biological source

human colon

growth mode

Semi-adherent

karyotype

Not specified

morphology

Predominantly round, refractile

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human colon carcinoma

Cell Line Description

COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.

Culture Medium

RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculture Routine

Maintain cultures between 3-9x105 cells/ml 5% CO2, 37°C. Any attached cells can be dislodged with trypsin/EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Certificate of Origin

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