Cell Line Origin
Human colon carcinoma
Cell Line Description
COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.
RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Maintain cultures between 3-9x105 cells/ml 5% CO2, 37°C. Any attached cells can be dislodged with trypsin/EDTA.
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