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BE11 (Early)

93040121

biological source

rat brain

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat, BDIX, foetal brain

Cell Line Description

The BE11 culture was derived 2 days after a single injection of buffer into pregnant rats. The cells do not form colonies in agar and are not tumourigenic in BDIX rats. Plasminogen activator activity is barely detectable. They are suitable controls for BE10 (Early) cells (93040118).

Application

Study of carcinogenesis

Culture Medium

DMEM + 2mM Glutamine + 15-20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C. Cultures should reach 2x1,000,000 cells per 25cm2 flask. Subculture weekly.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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