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3T3-MSV

90092701

biological source

mouse embryo

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Growth factor

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Product has lost contact inhibition

Cell Line Origin

Mouse Swiss 3T3 embryo, Moloney-MSV transformed

Cell Line Description

This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.

Application

Cell growth studies

Culture Medium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Certificate of Origin

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