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Cell Line Origin
Mouse Swiss 3T3 embryo, Moloney-MSV transformed
Cell Line Description
This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.
Cell growth studies
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.
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