All Photos(1)

DHD/K12/TRb

90062901

biological source

rat colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Rat colonic carcinoma

Cell Line Description

This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.

Application

Metastasis studies

Culture Medium

DMEM + F10 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or 2% Rat Serum.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

Certificate of Origin

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