Cell Line Origin
Mouse rectum carcinoma
Cell Line Description
From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Cells grow in clumps.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.