Cell Line Origin
Human squamous carcinoma of the tongue
Cell Line Description
25 year old male. Growth is enhanced by use of a feeder layer of X-irradiated STO cells.
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 ug/ml hydrocortisone + 0.5mM sodium pyruvate.
Split confluent cultures 1:3 to 1:6 using trypsin/EDTA; 5% CO2; 37°C.
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