MilliporeSigma
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A2

89030316

biological source

hamster ovary

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Ornithine decarboxylase

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Hamster Chinese ovary

Cell Line Description

Overproduces ornithine decarboxylase in response to polyamine deprivation.

Application

Enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + Ham′s F12 (without Putrescine) (1:1) + 0.1% BSA.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 minutes or until cells detach. Culture cells at 37°C; 5% CO2. Pre-soak flasks in porcine

Other Notes

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