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COLO 320DM

87061205

biological source

human colon

growth mode

Suspension

karyotype

Hyperdiploid, modal no. 53

morphology

Round & Refractile

products

Serotonin, epinephrine, NPP, Adrenocorticotropic hormone (ACTH) and PTH

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Isolated from a moderately undifferentiated adenocarcinoma from the sigmoid colon of a 55 year old Caucasian female. The cells have double minute (DM) chromosomes.

Application

Hormone studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 8,9
TPOX: 8,9
vWA: 15,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. may semi-adhere to culture flask.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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