P3X63Ag8

85011401, mouse skin, Lymphoblast

• UNSPSC Code: 41106514

Properties

• product name: P3X63Ag8, 85011401
• biological source: mouse skin
• growth mode: Suspension
• karyotype: Aneuploid
• morphology: Lymphoblast
• products: Immunoglobulin G (IgG1) (kappa)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse myeloma

Cell Line Description

Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20μg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.

Application

Fusion partner

Culture Medium

DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C. Cells will achieve maximum cell density of 5x100,000 cells/ml.

Other Notes

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