Cell Line Origin
Human colorectal adenocarcinoma, well differentiated, Dukes′ stage C
Cell Line Description
The C32 cell line was established from a 66-year old female patient with a well differentiated adenocarcinoma of the colon classified as Dukes′ stage C.
Iscove′s Modified Dulbecco′s Medium, + 10% FBS + 2 mM Glutamine
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
C32 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.